A new extract of the plant calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation
| Autor: | Eva Jiménez-Medina, Angel M. Garcia-Lora, Antonia Collado, Laura Paco, Federico Garrido, Ignacio Algarra |
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| Přispěvatelé: | [Jiménez-Medina,E, Garcia-Lora,A, Paco,L, Garrido,F] Servicio de Análisis Clínicos e Inmunologia, Hospital Universitario Virgen de las Nieves, Universidad de Granada, Granada, Spain. [Algarra,I] Departamento de Ciencias de la Salud, Universidad Jaén, Jaén, Spain. [Collado,A] Unidad de Investigación, Hospital Universitario Virgen de las Nieves, Granada, Spain., Studies were supported by a grant from Bomsund S.L. Malaga, Spain. |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Anatomy::Cells::Cells
Cultured::Cell Line::Cell Line Tumor [Medical Subject Headings] Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Asteraceae::Calendula [Medical Subject Headings] Pathology Cancer Research Trasplante de Neoplasias Fitoterapia Ratones Lymphocyte Apoptosis Lymphocyte Activation Extractos Vegetales Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Mice Calendula Cytotoxic T cell Lymphocytes biology Linfocitos Cell cycle lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Antineoplastic Humanos Phenomena and Processes::Immune System Phenomena::Immune System Processes::Lymphocyte Activation [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Complementary Therapies::Phytotherapy [Medical Subject Headings] medicine.anatomical_structure Oncology Research Article medicine.medical_specialty Ratones Desnudos Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Neoplasm Transplantation [Medical Subject Headings] Mice Nude Antineoplastic Agents In Vitro Techniques Neoplasm transplantation lcsh:RC254-282 In vivo Cell Line Tumor medicine Genetics Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes Mononuclear::Lymphocytes [Medical Subject Headings] Animals Humans Chemicals and Drugs::Complex Mixtures::Biological Agents::Plant Preparations::Plant Extracts [Medical Subject Headings] Lymphocyte activation Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] Activación de Linfocitos Cell growth business.industry Plant Extracts Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents [Medical Subject Headings] Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice Mutant Strains::Mice Nude [Medical Subject Headings] Plant extracts Antineoplásicos biology.organism_classification In vitro Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis [Medical Subject Headings] Línea Celular Tumoral Cell culture Calendula officinalis Animales Cancer research business Neoplasm Transplantation Phytotherapy |
| Zdroj: | BMC Cancer BMC Cancer, Vol 6, Iss 1, p 119 (2006) Digibug. Repositorio Institucional de la Universidad de Granada instname |
| Popis: | Background Phytopharmacological studies of different Calendula extracts have shown anti-inflamatory, anti-viral and anti-genotoxic properties of therapeutic interest. In this study, we evaluated the in vitro cytotoxic anti-tumor and immunomodulatory activities and in vivo anti-tumor effect of Laser Activated Calendula Extract (LACE), a novel extract of the plant Calendula Officinalis (Asteraceae). Methods An aqueous extract of Calendula Officinalis was obtained by a novel extraction method in order to measure its anti-tumor and immunomodulatory activities in vitro. Tumor cell lines derived from leukemias, melanomas, fibrosarcomas and cancers of breast, prostate, cervix, lung, pancreas and colorectal were used and tumor cell proliferation in vitro was measured by BrdU incorporation and viable cell count. Effect of LACE on human peripheral blood lymphocyte (PBL) proliferation in vitro was also analyzed. Studies of cell cycle and apoptosis were performed in LACE-treated cells. In vivo anti-tumor activity was evaluated in nude mice bearing subcutaneously human Ando-2 melanoma cells. Results The LACE extract showed a potent in vitro inhibition of tumor cell proliferation when tested on a wide variety of human and murine tumor cell lines. The inhibition ranged from 70 to 100%. Mechanisms of inhibition were identified as cell cycle arrest in G0/G1 phase and Caspase-3-induced apoptosis. Interestingly, the same extract showed an opposite effect when tested on PBLs and NKL cell line, in which in vitro induction of proliferation and activation of these cells was observed. The intraperitoneal injection or oral administration of LACE extract in nude mice inhibits in vivo tumor growth of Ando-2 melanoma cells and prolongs the survival day of the mice. Conclusion These results indicate that LACE aqueous extract has two complementary activities in vitro with potential anti-tumor therapeutic effect: cytotoxic tumor cell activity and lymphocyte activation. The LACE extract presented in vivo anti-tumoral activity in nude mice against tumor growth of Ando-2 melanoma cells. |
| Databáze: | OpenAIRE |
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