Activation of NMDA receptors drives action potentials in superficial dorsal horn from neonatal rats
| Autor: | Pier Cosimo Magherini, Rita Bardoni, Amy B. MacDermott |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Patch-Clamp Techniques
Action Potentials Kainate receptor Stimulation pain spinal cord patch-clamp AMPA receptor Biology Neurotransmission In Vitro Techniques Bicuculline Receptors N-Methyl-D-Aspartate GABA Antagonists medicine Animals Long-term depression General Neuroscience Excitatory Postsynaptic Potentials Glycine Agents Neural Inhibition Strychnine Electric Stimulation Rats nervous system Animals Newborn Spinal Cord Synapses NMDA receptor Spinal Nerve Roots Neuroscience Ion channel linked receptors medicine.drug |
| Zdroj: | Neuroreport. 11(8) |
| ISSN: | 0959-4965 |
| Popis: | We have investigated the role of NMDA receptors in mediating synaptic transmission in spinal cord lamina II over the first 2 weeks of postnatal development. High intensity root stimulation evoked D-APV-sensitive slow synaptic activity in lamina II neurons that drove action potential firing. This NMDA receptor-mediated activity was enhanced when bicuculline and strychnine were used to block synaptic inhibition. When activated by repetitive focal stimulation, synaptic activity mediated by NMDA receptors alone drove action potential firing. NMDA receptors were also able to drive action potential firing at synapses where AMPA receptors were present but blocked. Our data show that in lamina II of the dorsal horn, NMDA receptors significantly affect neuronal excitability even in the absence of co-activation of AMPA receptors. |
| Databáze: | OpenAIRE |
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