Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells*
| Autor: | Hai-Yan Liu, Hai-Dong Zhang, Yuan-Yuan Chen, Zhong-Dong Wang, Fan-Yong Qu, Zhang-Shen Ran |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Carcinoma Hepatocellular Cell Survival Green Fluorescent Proteins Biology General Biochemistry Genetics and Molecular Biology Article Green fluorescent protein 03 medical and health sciences 0302 clinical medicine Western blot Cell Movement Genes Reporter Internal medicine Cell Line Tumor microRNA medicine Humans Neoplasm Invasiveness General Pharmacology Toxicology and Pharmaceutics neoplasms Early Growth Response Protein 3 Cell Proliferation Reporter gene General Veterinary medicine.diagnostic_test Liver Neoplasms Computational Biology General Medicine Hep G2 Cells medicine.disease Phenotype digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Plasmids |
| Popis: | Objective: Hepatocellular carcinoma (HCC) is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. MicroRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 (miR-718) in the malignant phenotype of HCC cells and its possible role in HCC diagnosis. Methods: Here we first used a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, Transwell migration and invasion assays, and colony formation assay to evaluate the impact of miR-718 on the malignant phenotypes of HCC cells. Then, we used bioinformatic methods to predict the target gene of miR-718 and used green fluorescence protein (GFP) reporter assay, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) to validate the regulation relationship. Finally, we determined the role of the target gene in the HCC phenotype. Results: We found that the expression of miR-718 was significantly reduced in various HCC cell lines and HCC tissues. Re-expression of miR-718 significantly reduced the cellular viability and colony formation ability as well as inhibited the migration and invasion abilities of HCC cell lines. Early growth response protein 3 (EGR3) is a direct target of miR-718 and is negatively regulated by miR-718. EGR3 could increase the viability and proliferation of HCC cells, and promot the migration and invasion of HCC cells. Conclusions: miR-718 acts as a tumor suppressive microRNA in HCC via regulating the expression of EGR3, which may provide a new diagnostic marker and treatment target for HCC. |
| Databáze: | OpenAIRE |
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