Detection and Characterization of Membrane-Bound Selenoproteins in the Kidney of the Rat
| Autor: | Barbara Hoppe, H. Bertelsmann, M. Kühbacher, Dietrich Behne, A. Graebert, A. Kyriakopoulos |
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| Rok vydání: | 2002 |
| Předmět: |
Antioxidant
medicine.medical_treatment Selenium Radioisotopes chemistry.chemical_element Kidney General Biochemistry Genetics and Molecular Biology Selenium History and Philosophy of Science In vivo medicine Animals Amino Acids Rats Wistar Selenoproteins integumentary system Molecular mass General Neuroscience Proteins Rats Electrophoresis Membrane chemistry Biochemistry Microsome Autoradiography Cell fractionation |
| Zdroj: | Annals of the New York Academy of Sciences. 973:289-292 |
| ISSN: | 1749-6632 0077-8923 |
| DOI: | 10.1111/j.1749-6632.2002.tb04652.x |
| Popis: | The essential trace element selenium is of fundamental importance to human biology. Information on the membrane-bound selenoproteins was obtained by labeling of rats in vivo with 75Se-selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS-PAGE, two-dimensional electrophoresis, and selenium detection by autoradiography. In this way, in addition to already known membrane-bound selenoproteins three novel selenoproteins in the molecular mass range of 38–40, 18, and 12–13 kDa were detected in the microsomal membranes. |
| Databáze: | OpenAIRE |
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