Variation in concentration of prion protein in the peripheral blood of patients with variant and sporadic Creutzfeldt-Jakob disease detected by dissociation enhanced lanthanide fluoroimmunoassay and flow cytometry
| Autor: | Marc Turner, Ian MacGregor, Tim Fagge, Mark Head, James W. Ironside, G. Robin Barclay |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Pathology medicine.medical_specialty animal diseases Fluoroimmunoassay Immunology Lanthanoid Series Elements Sensitivity and Specificity Creutzfeldt-Jakob Syndrome Flow cytometry Central nervous system disease Degenerative disease mental disorders medicine Humans Immunology and Allergy PrPC Proteins Platelet Prion protein Whole blood medicine.diagnostic_test business.industry Age Factors Hematology Flow Cytometry medicine.disease Peripheral blood nervous system diseases business |
| Zdroj: | Transfusion. 45:504-513 |
| ISSN: | 1537-2995 0041-1132 |
| DOI: | 10.1111/j.0041-1132.2005.04342.x |
| Popis: | BACKGROUND: A highly sensitive dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) and flow cytometry techniques have previously been developed and employed to characterize soluble cellular prion protein (PrPc) expression in whole blood and separated components from healthy adult blood donors. No previous studies with these techniques have evaluated the concentration and expression of PrP in the blood of patients with variant Creutzfeldt-Jakob disease (vCJD). STUDY DESIGN AND METHODS: For blood from vCJD patients, sporadic CJD (sCJD) patients, non-CJD neurological controls, and healthy adults, PrPc was measured by DELFIA and cell-associated PrP was measured by flow cytometry. RESULTS: DELFIA analysis identified a significant reduction in the concentration of PrPc in the whole blood of vCJD (p = 0.012) and non-CJD neurological patients (p = 0.0004) compared with healthy adults. A significant elevation was found in plasma PrPc in sCJD patients compared with healthy adult (p = 0.022) and neurological controls (p = 0.050). Flow cytometry found no significant differences between groups in expression of PrP on platelets and lymphocytes, nor in sensitivity of cellular PrP to proteinase K. Neurological controls show significantly less PrP on red cells than healthy adults. CONCLUSION: There are differences in free and cell-associated PrP found in blood of CJD patients and control groups, some of which might be useful with other tests in disease profiling as an aid to diagnoses. |
| Databáze: | OpenAIRE |
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