PNA lectin for purifying mouse acinar cells from the inflamed pancreas
| Autor: | George K. Gittes, Shane Fischbach, Zewen Song, Philip Nebres, Krishna Prasadan, Ray Zimmerman, Joseph Fusco, David Ricks, Xiangwei Xiao, Chiyo Shiota, Sohail Z. Husain |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Peanut agglutinin Cell type Enteroendocrine cell Acinar Cells Cell Separation Article Mice Peanut Agglutinin 03 medical and health sciences Agglutinin Pancreatic cancer medicine Acinar cell Animals Pancreas Multidisciplinary biology Lectin Flow Cytometry medicine.disease Molecular biology 030104 developmental biology medicine.anatomical_structure Pancreatitis Immunology biology.protein |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Better methods for purifying human or mouse acinar cells without the need for genetic modification are needed. Such techniques would be advantageous for the specific study of certain mechanisms, such as acinar-to-beta-cell reprogramming and pancreatitis. Ulex Europaeus Agglutinin I (UEA-I) lectin has been used to label and isolate acinar cells from the pancreas. However, the purity of the UEA-I-positive cell fraction has not been fully evaluated. Here, we screened 20 widely used lectins for their binding specificity for major pancreatic cell types and found that UEA-I and Peanut agglutinin (PNA) have a specific affinity for acinar cells in the mouse pancreas, with minimal affinity for other major pancreatic cell types including endocrine cells, duct cells and endothelial cells. Moreover, PNA-purified acinar cells were less contaminated with mesenchymal and inflammatory cells, compared to UEA-I purified acinar cells. Thus, UEA-I and PNA appear to be excellent lectins for pancreatic acinar cell purification. PNA may be a better choice in situations where mesenchymal cells or inflammatory cells are significantly increased in the pancreas, such as type 1 diabetes, pancreatitis and pancreatic cancer. |
| Databáze: | OpenAIRE |
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