Quantitative features and clinical significance of two subpopulations of AChR-specific CD4+ T cells in patients with myasthenia gravis
| Autor: | Zhidong Huang, Changyi Ou, Xiaoxi Liu, Huan Huang, Weibin Liu, Pei Chen, Yaru Lu, Li Qiu, Qian Ma, Zhongqiang Lin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult CD4-Positive T-Lymphocytes Male animal structures Adolescent Immunology 03 medical and health sciences Young Adult 0302 clinical medicine Myasthenia Gravis medicine Immunology and Allergy Humans Clinical significance In patient Receptors Cholinergic Acetylcholine receptor Autoantibodies business.industry ELISPOT Antibody titer Autoantibody Middle Aged Th1 Cells musculoskeletal system medicine.disease Myasthenia gravis Acetylcholine 030104 developmental biology Biomarker (medicine) Th17 Cells Female business 030215 immunology |
| Zdroj: | Clinical immunology (Orlando, Fla.). 216 |
| ISSN: | 1521-7035 |
| Popis: | Acetylcholine receptor (AChR)-specific CD4+ T cells play a driving role in myasthenia gravis (MG) by regulating the production of autoantibodies. However, the quantitative features of AChR-specific T cells and their clinical significance in MG are unclear. In this study, we adopted standard and cultured enzyme-linked immunosorbent spot (ELISPOT) assays to quantify subpopulations of AChR-specific CD4+ T cells in MG patients, and evaluate their correlation with clinical characteristics. The results showed that Th1- and Th17-AChR-specific CD4+ T cells were detectable by standard and cultured ELISPOT assay respectively, with higher levels observed in MG patients comparing with healthy controls. The number of Th17-AChR-specific CD4+ T cells was positively correlated with anti-AChR antibody titer and quantitative MG score and may have latent capacity to reflect responses to immunosuppressants. These results highlight the differences in quantitative features of AChR-specific CD4+ T cells and imply Th17-AChR-specific CD4+ T cells can serve as a biomarker in MG. |
| Databáze: | OpenAIRE |
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