CD25+ T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses
| Autor: | Thaís Helena Gasparoto, João Santana da Silva, Rodrigo Nalio Ramos, Gustavo Pompermaier Garlet, Karen A. Cavassani, Tatiana Salles de Souza Malaspina, Carine Ervolino de Oliveira, Eduardo Bertoli Belai, Ana Paula Campanelli |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Tumor microenvironment Mice Inbred BALB C T-Lymphocytes Interleukin-2 Receptor alpha Subunit chemical and pharmacologic phenomena General Medicine T lymphocyte Biology Lymphocyte Depletion Interleukin 21 Mice Immune system Epidermoid carcinoma Immunology Carcinoma Squamous Cell Animals Cytokines Tumor necrosis factor alpha Female IL-2 receptor IMUNOLOGIA CD8 |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1460-2180 |
| Popis: | Squamous cell carcinoma (SCC) constitutes a microenvironment that could modulate the antitumor immune response. Also, tumor-infiltrating lymphocytes are believed to play complex regulatory roles in antitumor immunity against SCC. The presence of regulatory T cells (Tregs) has been associated with the suppression of tumor-reactive T cells. However, the underlying mechanism for this T cell dysfunction is not clear. We used a multistage model of SCC to examine the role of Treg cells during tumor development. 7,12-dimethylbenz[a]-anthracene/phorbol 12-myristate 13-acetate treatment and systemic depletion of Treg cells using an anti-CD25 monoclonal antibody (PC61) resulted in a decrease in the number and incidence of papilloma. Furthermore, CD25 depletion increased the proportion of CD8 + and CD4 + T cells that were isolated from tumor lesions. The levels of interleukin (IL)-1β, IL-10, IL-12, IL-13, interferon-γ, transforming growth factor-β and tumor necrosis factor-α, but not IL-17, were increased in the tumor microenvironment after Treg depletion. Therefore, our results indicated involvement of CD25 + T cells in SCC development and in the suppression of the inflammatory immune response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|