Cytochemistry of Oxidoreductases in Spermatozoa: The Technique Revisited
| Autor: | F. Hrudka |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Urology NADP metabolism Biology Endocrinology Species Specificity Oxidoreductase Animals Humans NADH NADPH Oxidoreductases False Negative Reactions Incubation NADH Tetrazolium Reductase chemistry.chemical_classification Formazans Histocytochemistry Nitroblue Tetrazolium General Medicine Spermatozoa Sperm Mitochondria Biochemistry chemistry Cytochemistry Oxidoreductases NADP Semen Preservation |
| Zdroj: | Andrologia. 11:337-353 |
| ISSN: | 1439-0272 0303-4569 |
| Popis: | A revised cytochemical procedure for demonstrating sperm oxidoreductases is presented in an attempt to standardize its use in comparative and experimental studies. Its detailed description includes improved incubation techniques, preparation of media, preservation of cytochemical activity on smears and evaluation of cytochemical preparations. Special attention is paid to the competing activities which may affect the specificity of the results. |
| Databáze: | OpenAIRE |
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