Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies

Autor: Nicola Cotugno, Alessandra Ruggiero, Francesco Bonfante, Maria Raffaella Petrara, Sonia Zicari, Giuseppe Rubens Pascucci, Paola Zangari, Maria Antonietta De Ioris, Veronica Santilli, E.C. Manno, Donato Amodio, Alessio Bortolami, Matteo Pagliari, Carlo Concato, Giulia Linardos, Andrea Campana, Daniele Donà, Carlo Giaquinto, Petter Brodin, Paolo Rossi, Anita De Rossi, Paolo Palma, Stefania Bernardi, Lorenza Romani, Paola Pansa, Sara Chiurchiú, Andrea Finocchi, Caterina Cancrini, Laura Lancella, Laura Cursi, Maia De Luca, Renato Cutrera, Libera Sessa, Elena Morrocchi, Chiara Medri, Lorenza Putignani, F.I. Calò Carducci, Patrizia D’Argenio, Marta Ciofi degli Atti, Carmen D’Amore, Livia Piccioni, Martina Di Giuseppe, Alessandro Jenkner, Carmela Giancotta, Andrzej Krzysztofiak
Rok vydání: 2021
Předmět:
CD4-Positive T-Lymphocytes
0301 basic medicine
Proteome
viruses
proteomic profiling
antigen-specific CD4 T 
Adaptive Immunity
Antibodies
Viral
Ab-mediated neutralization activity
anti-SARS-CoV-2 antibodies
antigen-specific B cells
antigen-specific CD4 T cells
COVID-19
pediatric COVID-19
SARS-CoV-2
Antibodies
Neutralizing
B-Lymphocytes
Child
Humans
Immunity
Humoral
Signal Transduction
Viral Load
0302 clinical medicine
Medicine
antigen-specific CD4 T cells
Viral
Lung
Neutralizing
Infectivity
biology
Humoral
Acquired immune system
Settore MED/38
Vaccination
Antibody
Viral load
COVID-19 Vaccines
Article
Antibodies
General Biochemistry
Genetics and Molecular Biology
Virus
Young Adult
03 medical and health sciences
Immunity
RNA
Messenger
BNT162 Vaccine
business.industry
biochemical phenomena
metabolism
and nutrition
030104 developmental biology
Immunization
Immunology
biology.protein
cells
business
030217 neurology & neurosurgery
Zdroj: Cell Reports
ISSN: 2211-1247
Popis: As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies.
Graphical abstract
Cotugno et al. show that neutralizing antibodies affect SARS-CoV-2 viral load in infected children. Antigen-specific B and T cells are positively associated with virus neutralization. This information provides a basis for defining SARS-CoV-2 adaptive responses in children.
Databáze: OpenAIRE
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