Identification and characterization of a functional receptor for interferon-gamma on a megakaryocytic cell line
Autor: | André Capron, Gilles Merlin, J P Kusnierz, Véronique Pancré, Didier Monté, Sheryl M. Greenberg, Claude Auriault, Jeanne Wietzerbin |
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Rok vydání: | 1991 |
Předmět: |
Immunology
Cell Biology Platelet membrane glycoprotein Biochemistry Cell Line Iodine Radioisotopes Interferon-gamma Interferon medicine Humans Interferon gamma RNA Messenger Receptors Immunologic Receptor Receptors Interferon Messenger RNA Cell growth Cell Biology Hematology Blotting Northern Precipitin Tests Molecular biology Recombinant Proteins Kinetics medicine.anatomical_structure Cell culture Megakaryocytes Protein Binding medicine.drug |
Zdroj: | Blood. 78:2062-2069 |
ISSN: | 1528-0020 0006-4971 |
Popis: | We have previously shown that human interferon-gamma (Hu-IFN-gamma) induces platelets to become efficient effector cells, capable of killing young larvae of the parasite Schistosoma mansoni. Recently, binding sites for IFN-gamma on platelets have been characterized. We show here the presence of high-affinity receptors for IFN-gamma on the surface of the human megakaryocytic Dami cell line. Scatchard analysis indicated the presence of about 11,000 binding sites per cell, with a kd of 3 +/- 0.5 x 10(-10) mol/L; the apparent molecular weight of the receptor was 90 Kd. Receptor-bound 125I Hu-recombinant IFN-gamma was rapidly internalized and degraded when the temperature was increased from 4 degrees C to 37 degrees C. The half-life of this receptor was about 7 hours, and pretreatment of cells with IFN-gamma or phorbol myristate acetate had very little effect on the surface receptor number and no detectable effect on IFN-gamma receptor messenger RNA (mRNA) expression. The receptor was functional, because 24 hours of treatment with IFN-gamma led to the increase of HLA class I mRNA expression and to the initiation of HLA class II mRNA expression. These effects were selective because platelet glycoprotein Ib, IIb, or IIIa mRNA expression and cell proliferation were unaffected. |
Databáze: | OpenAIRE |
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