Anti-Pneumococcal Vaccine-Induced Cellular Immune Responses in Post-Traumatic Splenectomized Individuals
| Autor: | Ayse Semra Gureser, Ozgur Tosun, Umut Gazi, Ayşegül Taylan Özkan, İbrahim Tayfun Şahiner, Mete Dolapci, Djursun Karasartova |
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| Přispěvatelé: | Hitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Cellular immunity Immunology Pneumococcal Infections Pneumococcal Vaccines Interferon-gamma Young Adult 03 medical and health sciences chemistry.chemical_compound Postoperative Complications 0302 clinical medicine Immune system Humans Immunology and Allergy Medicine 030212 general & internal medicine Cell Proliferation Immunity Cellular business.industry Vaccination Cell Differentiation Carboxyfluorescein succinimidyl ester Cellular Immunity Middle Aged Th1 Cells Virology Pneumococcal polysaccharide vaccine Streptococcus pneumoniae 030104 developmental biology Immunization Pneumococcal vaccine chemistry Humoral immunity Splenectomy Th17 Cells Wounds and Injuries Female Interleukin-4 business |
| Zdroj: | Journal of Clinical Immunology. 37:388-396 |
| ISSN: | 1573-2592 0271-9142 |
| Popis: | Splenectomy is associated with increased risk of overwhelming post-splenectomy infections despite proper anti-pneumococcal vaccination. As most studies concentrated on vaccination-induced humoral immunity, the cellular immune responses triggered in splenectomized patients are not yet well studied. The present study aims to investigate this area as it can contribute to the development of more effective vaccination strategies.Five healthy and 14 splenectomized patients were vaccinated with pneumococcal conjugate polysaccharide vaccine (PCV) followed by pneumococcal polysaccharide vaccine according to the guidelines established by Advisory Committee on Immunization Practices. PBMC samples collected 0, 8, and 12 weeks after PCV immunization were in vitro stimulated with PCV. Levels of lymphoproliferation, T-H cell differentiation, and cytokine release were assessed by carboxyfluorescein succinimidyl ester labeling, intracellular cytokine staining, and ELISA, respectively.While T(H)1-dominated immune response was detected in both groups, asplenic individuals generated significantly lower levels of T(H)1 cells following in vitro stimulation. Similarly, levels of IFN-gamma, IL-4, and IL-17 release and lymphoproliferation were significantly lower in asplenic patients.According to our data, splenectomy negatively influences the levels of PCV-induced lymphoproliferation, T(H)1 differentiation, and cytokine release. Besides, PCV failed to induce T(H)17-dominant immune response which is crucial for protection against extracellular pathogens. |
| Databáze: | OpenAIRE |
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