High-volume workflow and performance comparisons for Chlamydia trachomatis and Neisseria gonorrhoeae testing using automated molecular platforms
| Autor: | Elizabeth M. Marlowe, Andre Frontzek, Daniela Winkens, Gudrun Aretzweiler, Dana Duncan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA Bacterial 030106 microbiology Chlamydia trachomatis medicine.disease_cause Workflow Laboratory lcsh:Infectious and parasitic diseases 03 medical and health sciences Gonorrhea Automation 0302 clinical medicine McNemar's test Workflow analysis Significance testing medicine Screening programs Humans lcsh:RC109-216 030212 general & internal medicine Sexually transmitted diseases business.industry Significant difference Nucleic acid amplification technique Chlamydia Infections Neisseria gonorrhoeae Nucleic acid amplification techniques Infectious Diseases Body fluids Sensitivity and specificity Female Reagent Kits Diagnostic Nuclear medicine business Research Article |
| Zdroj: | BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-5 (2019) BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| Popis: | Background The global burden of sexually transmitted infections (STIs) is high and there have been reports of increasing chlamydial and gonorrheal infections. High-volume screening programs for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are an important component of STI control. This study evaluated the high-volume workflow and performance of the cobas® CT/NG assay for use on the automated Roche cobas® 6800 system, with the cobas p 480 instrument for pre-analytics, compared with the Aptima Combo 2 assay on the Hologic Panther system. Methods High-volume workflow and performance were evaluated using paired female urine specimens. Workflow analysis (n = 376) included hands-on time (HoT), number of manual interventions, and time to first and last results. For performance assessment, paired results from the cobas CT/NG and Aptima Combo 2 assays, for both CT and NG, were compared and two-sided 95% confidence intervals calculated to provide estimates of positive percent agreement (PPA), negative percent agreement (NPA), and overall percent agreement (OPA) between the tests. McNemar’s test was used for significance testing. Results Pre-analytical preparations and system start-up on the cobas 6800 system required 00:27:38 (hr:min:sec) HoT whilst the Panther system required 00:30:43. The cobas 6800 system required eight interactions and 00:43:59 HoT to process 376 samples. The Panther system required six interactions and 00:39:10 HoT. Time to first results was 02:53:00 on the cobas c6800 system for 96 samples and 03:28:29 on the Panther system for five samples. The cobas 6800 system delivered all 376 results 3 h faster than the Panther system (07:45:26 and 10:47:30, respectively). The performance correlation between both assays was high (PPA, NPA and OPA > 99% for both CT and NG). McNemar’s test revealed no statistically significant difference between the assays. Conclusion For high-volume automated CT/NG testing, both the cobas 6800 system and Panther system provided accurate results. Although less manual intervention steps were needed for the Panther system, improved turnaround time was obtained with the cobas 6800 system with less risk for contamination. The additional testing capacity on the cobas 6800 system would allow a growing service to deliver more results in a single shift. |
| Databáze: | OpenAIRE |
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