Stimulating Duddingtonia flagrans chlamydospore production through dehydration
| Autor: | Justin Blair, Amy S. Biddle |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Nematoda Fungus Biology Pasture Chlamydospore Feces 03 medical and health sciences Animal science food Duddingtonia Helminth medicine Animals Agar Anthelmintic Dehydration Nematode Infections Pest Control Biological Parasite Egg Count 030304 developmental biology Duddingtonia flagrans 0303 health sciences geography geography.geographical_feature_category General Veterinary Parafilm Diagnostic Tests Routine 030306 microbiology fungi General Medicine Spores Fungal medicine.disease biology.organism_classification Spore Helminthology - Original Paper Infectious Diseases Larva Biological control Insect Science Chlamydospore production Parasitology medicine.drug |
| Zdroj: | Parasitology Research |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Duddingtonia flagrans is a nematode-trapping fungus that has shown promising results as a tool to combat parasitic nematode infections in livestock. The fungus interrupts the parasitic lifecycle by trapping and killing larval stages on pasture to prevent re-infection of animals. One barrier to the fungus’ commercial use is scaling up production of the fungus, and specifically of chlamydospores, which survive the digestive tract to grow in fecal pats on pasture, thus have potential as a feed through anthelmintic. The purpose of this study was to evaluate the effect of dehydration on sporulation of the fungus. Disks of Duddingtonia flagrans type strain (ATCC® 13423™) were grown on 17% cornmeal agar for 26 days at 30 °C, then split into three groups; dried quickly at 38 °C and 37% humidity over 48 h (“incubated”), dried more slowly at 24 °C and 55% humidity over 10 days (“air-dried”), or kept at 30 °C and sealed with parafilm to prevent loss of moisture as a control (“wet”). Half of each dried culture was resuspended in water, then heated to liquify and homogenized through vortexing. Spores were then counted in a Neubauer hematocytometer. Both the “air-dried” and “incubated” drying techniques yielded significantly more spores than the “wet” control (Welch’s two sample t test p values of .0359 and .0411, respectively). The difference in average chlamydospores per milliliter was insignificant between the two drying techniques, although a visual representation of the data shows less spore count variability in the “air-dried” technique. |
| Databáze: | OpenAIRE |
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