Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes
Autor: | Hammond, Timothy R, Dufort, Connor, Dissing-Olesen, Lasse, Giera, Stefanie, Young, Adam, Wysoker, Alec, Walker, Alec J, Gergits, Frederick, Segel, Michael, Nemesh, James, Marsh, Samuel E, Saunders, Arpiar, Macosko, Evan, Ginhoux, Florent, Chen, Jinmiao, Franklin, Robin JM, Piao, Xianhua, McCarroll, Steven A, Stevens, Beth |
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Přispěvatelé: | Franklin, Robin [0000-0001-6522-2104], Apollo - University of Cambridge Repository |
Rok vydání: | 2019 |
Předmět: |
Aging
Multiple Sclerosis glia injury Sequence Analysis RNA brain Longevity microglia Cell Differentiation Adaptation Physiological diversity Mice Inbred C57BL Mice single-cell RNA seq Brain Injuries Animals Humans activation demyelination heterogeneity Single-Cell Analysis development Demyelinating Diseases |
Popis: | Microglia, the resident immune cells of the brain, rapidly change states in response to their environment, but we lack molecular and functional signatures of different microglial populations. Here, we analyzed the RNA expression patterns of more than 76,000 individual microglia in mice during development, in old age, and after brain injury. Our analysis uncovered at least nine transcriptionally distinct microglial states, which expressed unique sets of genes and were localized in the brain using specific markers. The greatest microglial heterogeneity was found at young ages; however, several states-including chemokine-enriched inflammatory microglia-persisted throughout the lifespan or increased in the aged brain. Multiple reactive microglial subtypes were also found following demyelinating injury in mice, at least one of which was also found in human multiple sclerosis lesions. These distinct microglia signatures can be used to better understand microglia function and to identify and manipulate specific subpopulations in health and disease. |
Databáze: | OpenAIRE |
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