Journal of Medical Microbiology
| Autor: | Albert I. Ko, Cláudio Pereira Figueira, Adenizar D. Chagas-Junior, Mitermayer G. Reis, Flávia W. C. McBride, Daniel Abensur Athanazio, Marco Alberto Medeiros, Alan J. A. McBride |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Hamster Leptospirose Kidney Sensitivity and Specificity Microbiology law.invention Leptospira law Immunity Cricetinae Leptospiraceae Vacinas Bacterianas medicine Animals Leptospirosis Tissue Distribution Lung biology Models of Infection Animais Sensibilidade e Especificidade General Medicine biology.organism_classification medicine.disease Virology Bacterial vaccine Liver Bacterial Vaccines Recombinant DNA Female Leptospira interrogans |
| Zdroj: | Repositório Institucional da FIOCRUZ (ARCA) Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.014050-0 |
| Popis: | Texto completo: acesso restrito. p.1632-1637 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-17T14:33:03Z No. of bitstreams: 1 0.1099jmm.0.014050-0.pdf: 220400 bytes, checksum: a7d90c3e814de07b78e40307f7d81071 (MD5) Approved for entry into archive by Patricia Barroso(pbarroso@ufba.br) on 2013-09-17T17:28:49Z (GMT) No. of bitstreams: 1 0.1099jmm.0.014050-0.pdf: 220400 bytes, checksum: a7d90c3e814de07b78e40307f7d81071 (MD5) Made available in DSpace on 2013-09-17T17:28:49Z (GMT). No. of bitstreams: 1 0.1099jmm.0.014050-0.pdf: 220400 bytes, checksum: a7d90c3e814de07b78e40307f7d81071 (MD5) Previous issue date: 2010 In determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (κ=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (κ=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates. |
| Databáze: | OpenAIRE |
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