Specific interaction of the nonstructural protein NS1 of minute virus of mice (MVM) with [ACCA](2) motifs in the centre of the right-end MVM DNA palindrome induces hairpin-primed viral DNA replication
| Autor: | Rita Hörlein, Wolfgang Stremmel, Kurt Willwand, Adela Moroianu, Jean Rommelaere |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA Replication
DNA synthesis biology Base Sequence viruses Molecular Sequence Data DNA replication Palindrome biochemical phenomena metabolism and nutrition Viral Nonstructural Proteins biology.organism_classification Virus Replication Genome Virology Molecular biology chemistry.chemical_compound chemistry DNA Viral Minute Virus of Mice Sequence motif Minute virus of mice DNA Palindromic sequence Protein Binding |
| Zdroj: | The Journal of general virology. 83(Pt 7) |
| ISSN: | 0022-1317 |
| Popis: | The linear single-stranded DNA genome of minute virus of mice (MVM) is replicated via a double-stranded replicative form (RF) intermediate DNA. Amplification of viral RF DNA requires the structural transition of the right-end palindrome from a linear duplex into a double-hairpin structure, which serves for the repriming of unidirectional DNA synthesis. This conformational transition was found previously to be induced by the MVM nonstructural protein NS1. Elimination of the cognate NS1-binding sites, [ACCA]2, from the central region of the right-end palindrome next to the axis of symmetry was shown to markedly reduce the efficiency of hairpin-primed DNA replication, as measured in a reconstituted in vitro replication system. Thus, [ACCA]2 sequence motifs are essential as NS1-binding elements in the context of the structural transition of the right-end MVM palindrome. |
| Databáze: | OpenAIRE |
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