Validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis
| Autor: | D. Gall, P. Smith, P. Nicoletti, Luis Samartino, Klaus Nielsen, Fred M. Enright, Ana M. Vigliocco, B. Perez, A Dajer, Philip H. Elzer |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Swine
Enzyme-Linked Immunosorbent Assay Brucellaceae Sensitivity and Specificity Microbiology Brucellosis Antigen Agglutination Tests Direct agglutination test Fluorescence Polarization Immunoassay parasitic diseases medicine Animals Swine Diseases General Veterinary biology medicine.diagnostic_test Complement Fixation Tests General Medicine Assay sensitivity biology.organism_classification Complement fixation test medicine.disease Antibodies Bacterial Brucella Virology Molecular biology ROC Curve Immunoassay Fluorescence anisotropy |
| Zdroj: | Veterinary Microbiology. 68:245-253 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(99)00077-2 |
| Popis: | Sera from Canadian pigs (brucellosis free, n = 14 037) and sera from pigs infected with Brucella suis (n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. The results were analysed and assay sensitivity and specificity estimates were calculated. The sensitivity and specificity of the tests were as follows: the buffered antigen plate agglutination test, 77.1 and 96.9%; the complement fixation test (considering anticomplementary sera as negative), 93.3 and 95.5%; the complement fixation test (considering anticomplementary sera as positive), 58.1 and 99.9%; the indirect enzyme immunoassay, 94.0 and 97.9%; the competitive enzyme immunoassay, 90.8 and 96.6%; and the fluorescence polarization assay, 93.5 and 97.2%; respectively. It was concluded that the fluorescence polarization assay was a valuable asset to the diagnosis of porcine brucellosis because of its accuracy, ease of performance and relative cost. |
| Databáze: | OpenAIRE |
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