Detection of Candida albicans mRNA from formalin-fixed, paraffin-embedded mouse tissues by nested reverse transcription-PCR
| Autor: | Edward Balish, Peter J. Nicholas, David A. Schofield, Caroline Westwater, Emily E. Paulling |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Paraffin Embedding Reverse Transcriptase Polymerase Chain Reaction Histological Techniques RNA Mycology Biology biology.organism_classification Molecular biology Corpus albicans Reverse transcriptase Housekeeping gene Microbiology Reverse transcription polymerase chain reaction Mice Formaldehyde Gene expression Candida albicans Animals RNA Messenger Nested polymerase chain reaction |
| Zdroj: | Journal of clinical microbiology. 41(2) |
| ISSN: | 0095-1137 |
| Popis: | Histopathology archives represent a vast source of infectious disease specimens that can be used to elucidate important disease processes. In this report, we describe a method to detect Candida albicans gene expression from infected, formalin-fixed, paraffin-embedded mouse tissue. By use of glass beads to break open the fungal cells and proteinase K treatment, RNA was extracted routinely from tissue sections that had been fixed for up to 72 h. Upon reverse transcription of the RNA and nested PCR, the procedure detected C. albicans “housekeeping” and putative virulence genes. |
| Databáze: | OpenAIRE |
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