Microparticles from glycidylmethacrylated gelatin as cell carriers prepared in an aqueous two-phase system
| Autor: | Andreas Lendlein, Toralf Roch, Dunia M. Garcia Cruz, Axel T. Neffe |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
food.ingredient
Polymers and Plastics Chemistry Organic Chemistry Aqueous two-phase system General Physics and Astronomy 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Gelatin 0104 chemical sciences chemistry.chemical_compound Dextran food Photopolymer Cell culture Materials Chemistry Biophysics Photosensitizer Microparticle 0210 nano-technology Cell encapsulation |
| Zdroj: | Neffe, A.; Garcia Cruz, D.; Roch, T.; Lendlein, A.: Microparticles from glycidylmethacrylated gelatin as cell carriers prepared in an aqueous two-phase system. In: European Polymer Journal. Vol. 142 (2021) 110148. (DOI: /10.1016/j.eurpolymj.2020.110148) |
| ISSN: | 0014-3057 |
| Popis: | Encapsulation by polymeric biomaterials can provide mechanical protection of cells and shielding from the immune system of the host when implanted as cell therapy. At the same time, free exchange of nutrients and metabolites including bioactive molecules guiding regenerative processes is facilitated. Here, glycidylmethacrylated gelatin (GMA-gelatin) is explored as matrix material for adherent (L929 mouse fibroblasts) or non-adherent (Ramos blue) cells by an integrated process of shaping and chemical crosslinking. Microparticle formation was driven by a water-in-water-emulsion technique, which allowed simultaneous irradiation with light of 365 nm in the presence of the photosensitizer irgacure 2959. Suitable photopolymerization conditions were determined in experiments with GMA-gelatin and cells. More than 85% of the cells survived this procedure, and an encapsulation efficiency of up to 75 ± 2% was reached. Diffusivity of molecules up to a molar mass of 150 kg·mol−1 in the matrix was shown by the release of co-encapsulated FITC-labelled dextran. L929 as well as Ramos blue cells proliferated in the microparticle matrix after encapsulation and released enzymes that could be detected in the cell culture medium in an active form. L929 showed the ability to escape the particles over time. Altogether, the presented cell encapsulation system based on a material that is stable to hydrolytic degradation for several weeks is generally suitable for cell based therapy or in vitro test systems. |
| Databáze: | OpenAIRE |
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