SdiA, a Quorum-Sensing Regulator, Suppresses Fimbriae Expression, Biofilm Formation, and Quorum-Sensing Signaling Molecules Production in Klebsiella pneumoniae
| Autor: | Henrietta Venter, Ana Érika Inácio Gomes, Michelle Darrieux, Thaisy Pacheco, Nathália Maria Gonçalves Siqueira, Lúcio Fábio Caldas Ferraz, Lucas Assoni |
|---|---|
| Přispěvatelé: | Pacheco, Thaisy, Gomes, Ana Érika Inácio, Siqueira, Nathália Maria Gonçalves, Assoni, Lucas, Darrieux, Michelle, Venter, Henrietta, Ferraz, Lúcio Fábio Caldas |
| Rok vydání: | 2021 |
| Předmět: |
cell division
Microbiology (medical) Cell signaling Fimbria Homoserine Virulence Microbiology biofilm Virulence factor 03 medical and health sciences chemistry.chemical_compound Klebsiella pneumonia 030304 developmental biology 0303 health sciences 030306 microbiology Biofilm quorum sensing biochemical phenomena metabolism and nutrition SdiA regulator QR1-502 Cell biology Quorum sensing type 1 fimbriae chemistry Autoinducer |
| Zdroj: | Frontiers in Microbiology, Vol 12 (2021) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2021.597735 |
| Popis: | Klebsiella pneumoniae is a Gram-negative pathogen that has become a worldwide concern due to the emergence of multidrug-resistant isolates responsible for various invasive infectious diseases. Biofilm formation constitutes a major virulence factor for K. pneumoniae and relies on the expression of fimbrial adhesins and aggregation of bacterial cells on biotic or abiotic surfaces in a coordinated manner. During biofilm aggregation, bacterial cells communicate with each other through inter- or intra-species interactions mediated by signallng molecules, called autoinducers, in a mechanism known as quorum sensing (QS). In most Gram-negative bacteria, intra-species communication typically involves the LuxI/LuxR system: LuxI synthase produces N-acyl homoserine lactones (AHLs) as autoinducers and the LuxR transcription factor is their cognate receptor. However, K. pneumoniae does not produce AHL but encodes SdiA, an orphan LuxR-type receptor that responds to exogenous AHL molecules produced by other bacterial species. While SdiA regulates several cellular processes and the expression of virulence factors in many pathogens, the role of this regulator in K. pneumoniae remains unknown. In this study, we describe the characterization of sdiA mutant strain of K. pneumoniae. The sdiA mutant strain has increased biofilm formation, which correlates with the increased expression of type 1 fimbriae, thus revealing a repressive role of SdiA in fimbriae expression and bacterial cell adherence and aggregation. On the other hand, SdiA acts as a transcriptional activator of cell division machinery assembly in the septum, since cells lacking SdiA regulator exhibited a filamentary shape rather than the typical rod shape. We also show that K. pneumoniae cells lacking SdiA regulator present constant production of QS autoinducers at maximum levels, suggesting a putative role for SdiA in the regulation of AI-2 production. Taken together, our results demonstrate that SdiA regulates cell division and the expression of virulence factors such as fimbriae expression, biofilm formation, and production of QS autoinducers in K. pneumoniae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|