Transductional Analysis of Pseudomonas aeruginosa Methionineless Auxotrophs

Autor: T. W. Feary, D. H. Calhoun
Rok vydání: 1969
Předmět:
Zdroj: Journal of Bacteriology. 97:210-216
ISSN: 1098-5530
0021-9193
DOI: 10.1128/jb.97.1.210-216.1969
Popis: Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mutants and at least three among the cysteineless mutants. These studies suggested that the biosynthesis of methionine in P. aeruginosa is similar to that described in other microorganisms, although none of the mutants lacking the ability to methylate homocysteine grew with vitamin B 12 or S -adenosylmethionine.
Databáze: OpenAIRE