Enumeration of Chemoorganotrophic Carbonyl Sulfide (COS)-degrading Microorganisms by the Most Probable Number Method
| Autor: | Hiroyuki Ohta, Yoko Katayama, Takahiro Ogawa, Hiromi Kato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microorganism
carbonic anhydrase Colony Count Microbial Sulfur Oxides Soil Science chemistry.chemical_element Plant Science Volcanic Eruptions Forests 03 medical and health sciences chemistry.chemical_compound Most probable number soil microbes most probable number method Regular Paper Biomass Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology 030304 developmental biology Carbonyl sulfide 0303 health sciences biology Bacteria 030306 microbiology Sulfur cycle food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification Bacillales Sulfur Culture Media Biodegradation Environmental chemistry Environmental chemistry Soil water Water Microbiology carbonyl sulfide |
| Zdroj: | Microbes and Environments |
| ISSN: | 1347-4405 |
| Popis: | Carbonyl sulfide (COS) is the most abundant sulfur compound in the atmosphere, and, thus, is important in the global sulfur cycle. Soil is a major sink of atmospheric COS and the numerical distribution of soil microorganisms that degrade COS is indispensable for estimating the COS-degrading potential of soil. However, difficulties are associated with counting COS-degrading microorganisms using culture-dependent approaches, such as the most probable number (MPN) method, because of the chemical hydrolysis of COS by water. We herein developed a two-step MPN method for COS-degrading microorganisms: the first step for chemoorganotrophic growth that supported a sufficient number of cells for COS degradation in the second step. Our new MPN analysis of various environmental samples revealed that the cell density of COS-degrading microorganisms in forest soils ranged between 106 and 108 MPN (g dry soil)-1, which was markedly higher than those in volcanic deposit and water samples, and strongly correlated with the rate of COS degradation in environmental samples. Numerically dominant COS degraders that were isolated from the MPN-positive culture were related to bacteria in the orders Bacillales and Actinomycetales. The present results provide numerical evidence for the ubiquity of COS-degrading microbes in natural environments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|