Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes
| Autor: | M. Goreti F. Sales, Liliana A.A.N.A. Truta, Sónia Tarelho, Pedro Costa, André L. Castro, Helena M. Teixeira |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
Gas Clinical Biochemistry Pharmaceutical Science Tandem mass spectrometry Sensitivity and Specificity 01 natural sciences Analytical Chemistry Forensic Toxicology 03 medical and health sciences 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry Drug Discovery medicine Humans Selected ion monitoring 030216 legal & forensic medicine Solid phase extraction Spectroscopy Detection limit Solid-phase extraction Chromatography Gas Chromatography/Tandem Mass Spectrometry Chemistry Solid Phase Extraction 010401 analytical chemistry GC–MS/MS Reproducibility of Results Antidepressants Mianserin Antidepressive Agents Forensic toxicology 0104 chemical sciences Triple quadrupole mass spectrometer Whole blood Gas chromatography–mass spectrometry medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 128:496-503 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2016.06.027 |
| Popis: | Depression is among the most prevalent psychiatric disorders of our society, leading to an increase in antidepressant drug consumption that needs to be accurately determined in whole blood samples in Forensic Toxicology Laboratories. For this purpose, this work presents a new gas chromatography tandem mass spectrometry (GC–MS/MS) method targeting the simultaneous and rapid determination of 14 common Antidepressants in whole blood: 13 Antidepressants (amitriptyline, citalopram, clomipramine, dothiepin, fluoxetine, imipramine, mianserin, mirtazapine, nortryptiline, paroxetine, sertraline, trimipramine and venlafaxine) and 1 Metabolite (N-desmethylclomipramine). Solid-phase extraction was used prior to chromatographic separation. Chromatographic and MS/MS parameters were selected to improve sensitivity, peak resolution and unequivocal identification of the eluted analyte. The detection was performed on a triple quadrupole tandem MS in selected ion monitoring (SIM) mode in tandem, using electronic impact ionization. Clomipramine-D3 and trimipramine-D3 were used as deutered internal standards. The validation parameters included linearity, limits of detection, lower limit of quantification, selectivity/ specificity, extraction efficiency, carry-over, precision and robustness, and followed internationally accepted guidelines. Limits of quantification and detection were lower than therapeutic and subtherapeutic concentration ranges. Overall, the method offered good selectivity, robustness and quick response ( |
| Databáze: | OpenAIRE |
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