SiCTeC: an inexpensive, easily assembled Peltier device for rapid temperature shifting during single-cell imaging

Autor: Lillian Zhu, Benjamin D. Knapp, Kerwyn Casey Huang
Rok vydání: 2020
Předmět:
0301 basic medicine
Pathology and Laboratory Medicine
Stiffness
Open Science
0302 clinical medicine
Medicine and Health Sciences
Cell Cycle and Cell Division
Biology (General)
Materials
Escherichia Coli
General Neuroscience
Methods and Resources
Temperature
Software Engineering
Equipment Design
Microfluidic Analytical Techniques
Bacterial Pathogens
Phenotypes
Experimental Organism Systems
Cell Processes
Medical Microbiology
Physical Sciences
Engineering and Technology
Prokaryotic Models
Optoelectronics
Genetic Oscillators
Pathogens
Single-Cell Analysis
General Agricultural and Biological Sciences
Open Source Software
Escherichia
Computer and Information Sciences
Thermoelectric cooling
Materials science
QH301-705.5
Science Policy
Thermometers
Amorphous Solids
Materials Science
Material Properties
Microfluidics
Equipment
Biology
Heat sink
Research and Analysis Methods
Microbiology
Temperature measurement
General Biochemistry
Genetics and Molecular Biology
Computer Software
Heating
Stress (mechanics)
03 medical and health sciences
Model Organisms
Enterobacteriaceae
Control theory
Thermoelectric effect
Genetics
Mechanical Properties
Microbial Pathogens
Temperature control
Bacteria
General Immunology and Microbiology
Imaging Equipment
business.industry
Gut Bacteria
Organisms
Biology and Life Sciences
Cell Biology
Microcontroller
030104 developmental biology
Mixtures
Animal Studies
business
Gels
030217 neurology & neurosurgery
Zdroj: PLoS Biology
PLoS Biology, Vol 18, Iss 11, p e3000786 (2020)
DOI: 10.1101/2020.05.29.123158
Popis: Single-cell imaging, combined with recent advances in image analysis and microfluidic technologies, have enabled fundamental discoveries of cellular responses to chemical perturbations that are often obscured by traditional liquid-culture experiments. Temperature is an environmental variable well known to impact growth and to elicit specific stress responses at extreme values; it is often used as a genetic tool to interrogate essential genes. However, the dynamic effects of temperature shifts have remained mostly unstudied at the single-cell level, due largely to engineering challenges related to sample stability, heatsink considerations, and temperature measurement and feedback. Additionally, the few commercially available temperature-control platforms are costly. Here, we report an inexpensive (
The Single-Cell Temperature Controller (SiCTeC), made using inexpensive and accessible components, can maintain sample temperatures within 0.2°C from room temperature to 90°C, with upshifts and downshifts equilibrating within minutes. This study uses SiCTeC to explore the effects of extreme temperatures on Escherichia coli, and to probe the properties of temperature-sensitive cell-wall mutants.
Databáze: OpenAIRE
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