First detection of Leishmania major DNA in Sergentomyia (Splaeomyia) darlingi from cutaneous Leishmaniasis Foci in Mali

Autor: Pascal Del Giudice, Jérôme Depaquit, Christophe Ravel, Siaka Goita, Abdoulaye K. Kone, Siala Doumbo, Pascal Delaunay, Abdoulaye Djimde, Zohra Berdjane-Brouk, Ogobara K. Doumbo, Arezki Izri, Rémi N. Charrel, Adama Z. Diarra, Pierre Marty, Mahamadou A. Thera
Přispěvatelé: Emergence des Pathologies Virales (EPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département d'Epidémiologie des Affections parasitaires, Malaria Research and training center Université de Bamako, Mali, Université de Bamako, Biologie, Génétique et Pathologie des Pathogènes Eucaryotes (MIVEGEC-BioGEPPE), Pathogènes, Environnement, Santé Humaine (EPATH), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire de Parasitologie-Mycologie, Nice, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Université Côte d'Azur (UCA), Unité des Maladies infectieuses et tropicales, Hôpital Bonnet, Environnement, Santé, Sociétés (ESS), Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Veterinary medicine
[SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology
Sergentomyia (Spelaeomyia)
Sand flies
Protozoology
Mali
law.invention
0302 clinical medicine
law
Leishmania major
Phlebotomus
Leishmaniasis
Polymerase chain reaction
Leishmania
0303 health sciences
Multidisciplinary
biology
Zoonotic Diseases
3. Good health
Infectious Diseases
Veterinary Diseases
Medicine
Female
Research Article
Neglected Tropical Diseases
Old World
Science
030231 tropical medicine
Leishmaniasis
Cutaneous
Microbiology
03 medical and health sciences
Cutaneous leishmaniasis
parasitic diseases
medicine
Parasitic Diseases
Animals
Psychodidae
Biology
DNA sequence analysis
030304 developmental biology
DNA
DNA
Protozoan
biology.organism_classification
medicine.disease
Insect Vectors
Parastic Protozoans
Veterinary Science
Cutaneous Leishmaniasis Foci
Zdroj: PLoS ONE
PLoS ONE, 2012, 7 (1), pp.e28266. ⟨10.1371/journal.pone.0028266⟩
PLoS ONE, Vol 7, Iss 1, p e28266 (2012)
PLoS ONE, Public Library of Science, 2012, 7 (1), pp.e28266. ⟨10.1371/journal.pone.0028266⟩
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0028266⟩
Popis: BackgroundLeishmania major complex is the main causative agent of zoonotic cutaneous leishmaniasis (ZCL) in the Old World. Phlebotomus papatasi and Phlebotomus duboscqi are recognized vectors of L. major complex in Northern and Southern Sahara, respectively. In Mali, ZCL due to L. major is an emerging public health problem, with several cases reported from different parts of the country. The main objective of the present study was to identify the vectors of Leishmania major in the Bandiagara area, in Mali.Methodology/principal findingsAn entomological survey was carried out in the ZCL foci of Bandiagara area. Sandflies were collected using CDC miniature light traps and sticky papers. In the field, live female Phlebotomine sandflies were identified and examined for the presence of promastigotes. The remaining sandflies were identified morphologically and tested for Leishmania by PCR in the ITS2 gene. The source of blood meal of the engorged females was determined using the cyt-b sequence. Out of the 3,259 collected sandflies, 1,324 were identified morphologically, and consisted of 20 species, of which four belonged to the genus Phlebotomus and 16 to the genus Sergentomyia. Leishmania major DNA was detected by PCR in 7 of the 446 females (1.6%), specifically 2 out of 115 Phlebotomus duboscqi specimens, and 5 from 198 Sergentomyia darlingi specimens. Human DNA was detected in one blood-fed female S. darlingi positive for L. major DNA.ConclusionOur data suggest the possible involvement of P. duboscqi and potentially S. darlingi in the transmission of ZCL in Mali.
Databáze: OpenAIRE
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