Boronic acid disk diffusion for the phenotypic detection of polymerase chain reaction-confirmed, carbapenem-resistant, gram-negative bacilli isolates
| Autor: | Rasha Elsherif, Gamal Karrouf, Saad B. Almasaudi, Samyah Jastaniah, Steve Harakeh, Sanaa Elawady, Dalia Ibrahim Ismail |
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| Jazyk: | angličtina |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Imipenem Carbapenem Phenotypic screening Klebsiella pneumoniae 030106 microbiology Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Meropenem beta-Lactamases Disk Diffusion Antimicrobial Tests 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Drug Resistance Multiple Bacterial Gram-Negative Bacteria medicine Humans Pseudomonas aeruginosa Acinetobacter biology.organism_classification Boronic Acids Disk diffusion Enterobacteriaceae Anti-Bacterial Agents Boronic acid Phenotype Carbapenems chemistry Female Thienamycins Gram-Negative Bacterial Infections Research Article medicine.drug |
| Zdroj: | BMC Microbiology |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-016-0754-z |
| Popis: | Background The Middle East is regarded as a secondary reservoir for OXA-48 and New Delhi metallo-β-lactamase (NDM) carbapenemases. One of the main challenges in clinical microbiology diagnostics is the detection of carbapenemases. For this reason simple screening methods have been sought to detect carbapenemase producers to determine appropriate therapeutic measures and implement infection control interventions. The present study aimed to evaluate the efficacy of the modified Hodge test (MHT) and a boronic acid-based combined disk test using carbapenems as substrates for the phenotypic determination of OXA-48 and NDM type carbapenemases in 45 epidemiologically unrelated carbapenem-resistant clinical isolates of Klebsiella pneumoniae (13 isolates), Acinetobacter baumanii (20 isolates), and Pseudomonas aeruginosa (12 isolates). Results Boronic acid disk test using meropenem as substrate and 600 µg of 3- aminophenylboronic acid (APB) was the most sensitive method (83.33 %) for detection of OXA-48, while the most specific method was MHT (100 %). As regards NDM carbapenemase, boronic acid disk tests using imipenem and 600 µg of APB per disk, and meropenem with 300 or 600 µg of APB were the most sensitive methods (87.50 %), while the most specific method was the MHT (100 %). Conclusions The results of the present study indicate that phenotypic screening with the MHT and the boronic acid disk test may be used to detect OXA-48 and NDM carbapenemases in Gram-negative bacilli clinical isolates, and that these tests can be easily applied in tertiary care settings with minimal infrastructure. |
| Databáze: | OpenAIRE |
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