Alternative protein secretion: the Mam1 ABC transporter supports secretion of M-factor linked GFP in fission yeast
| Autor: | Martin Roland Jensen, Sven Müller, Søren Kjærulff |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Recombinant Fusion Proteins
Green Fluorescent Proteins Molecular Sequence Data Biophysics Heterologous ATP-binding cassette transporter Peptide Biochemistry Green fluorescent protein Schizosaccharomyces Secretion Amino Acid Sequence Protein Precursors Molecular Biology chemistry.chemical_classification biology fungi Transporter Cell Biology biology.organism_classification Yeast Cell biology chemistry Schizosaccharomyces pombe Intercellular Signaling Peptides and Proteins ATP-Binding Cassette Transporters Schizosaccharomyces pombe Proteins Peptides Sequence Alignment |
| Zdroj: | Biochemical and biophysical research communications. 338(4) |
| ISSN: | 0006-291X |
| Popis: | To examine whether the fission yeast Mam1 ABC transporter can be used for secretion of heterologous proteins, thereby bypassing the classical secretion pathway, we have analyzed chimeric forms of the M-factor precursor. It was demonstrated that GFP can be exported when fused to both the amino-terminal prosequence from mfm1 and a CaaX motif. This secretion was dependent on the Mam1 transporter and not the classical secretion pathway. The secretion efficiency of GFP, however, was relatively low and most of the reporter protein was trapped in the vacuolar membranes. Our findings suggest that the Mam1 ABC protein is a promiscuous peptide transporter that can accommodate globular proteins of a relatively large size. Furthermore, our results help in defining the sequences required for processing and secretion of natural M-factor. |
| Databáze: | OpenAIRE |
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