Induction of Lactogenic Binding Sites in the Liver of the Snell Dwarf Mouse
| Autor: | Sue C. Liu, Pietro M. Gullino, Richard A. Knazek |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Male
Pituitary gland medicine.medical_specialty Dwarfism Receptors Cell Surface Biology Mice Endocrinology Internal medicine medicine Animals Transplantation Homologous Binding site Dwarfism Pituitary Kidney Body Weight Homozygote Metabolism medicine.disease Prolactin Transplantation Thyroxine medicine.anatomical_structure Liver Growth Hormone Pituitary Gland Female Kidney capsule |
| Zdroj: | Endocrinology. 101:50-58 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-101-1-50 |
| Popis: | Lactogenic binding sites were shown to be absent in the livers of Snell dwarf mice (dw/dw). Phenotypically normal littermates (DW/?), however, bound oPRL with an apparent affinity constant, Ka, 15 × 109M−1 and possessed quantities of binding sites equivalent to 25 and 8 fmoles per mg liver protein in females and males, respectively. Lactogenic binding sites were induced in livers of dw/dw mice upon bans plantation of intact DW/? pituitaries under the kidney capsule, induction occurring in a biphasic manner. Detectable binding sites could not be reinduced by daily single oPRL injections into dw/dw after the transplanted pituitary had been excised. Intraperitoneal injections of bGH + oPRL + thyroxine (T4) every 4 or 8 h induced detectable binding sites that were maximally detectable 8–12 h after the final injection. Injection of bGH alone every 8 h for 2 days also induced the lactogenic binding sites in an amount approaching that of the female DW/?. DW/? males exhibited a 6.5-fold or 2-fold increase in the ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|