Corynebacterium kroppenstedtii in granulomatous mastitis: Analysis of formalin-fixed, paraffin-embedded biopsy specimens by immunostaining using low-specificity bacterial antisera and real-time polymerase chain reaction
| Autor: | Yutaka Tsutsumi, Michihiko Harada, Kiichiro Okamoto, Kouichiro Tagami, Yasushi Kuno, Koichi Kubouchi, Yasuyoshi Mizutani, Mari Fujii, Takahiko Sakuma |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty Tissue Fixation 030106 microbiology Granulomatous mastitis Corynebacterium Cross Reactions Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Antigen Antibody Specificity Formaldehyde Biopsy medicine Humans Granulomatous Mastitis Escherichia coli Antiserum Paraffin Embedding Treponema Corynebacterium Infections medicine.diagnostic_test General Medicine Middle Aged biology.organism_classification medicine.disease Antibodies Bacterial Immunohistochemistry Real-time polymerase chain reaction 030220 oncology & carcinogenesis Female Immunostaining |
| Zdroj: | Pathology International. 68:409-418 |
| ISSN: | 1320-5463 |
| Popis: | Granulomatous mastitis (GM) is a rare inflammatory disease of the post-lactation breast, clinically mimicking breast cancer. GM is microscopically characterized by formation of epithelioid granulomas and abscess (suppurative granulomas) with lipid droplet-centered inflammation. Corynebacterium kroppenstedtii (Ck) is known as a causative bacterium of GM, and identification of Ck infection within the lesion should thus be essential for confirming the diagnosis. In the present study, we analyzed formalin-fixed, paraffin-embedded (FFPE) biopsy specimens of a total of 18 GM lesions with immunostaining and real-time PCR for Ck genome. Widely cross-reactive rabbit antisera against Bacillus Calmette-Guerin (BCG), Bacillus cereus, Treponema pallidum and Escherichia coli were chosen. With real-time PCR, Ck genome was demonstrated in 7 of 18 GM lesions. Immunohistochemically, the low-specificity antisera reacted with the cytoplasm of phagocytes and/or granuloma-engulfed lipid droplets in 12 of 18 GM lesions. Antigenic positivity was observed in the following order: BCG > B. cereus > T. pallidum > E. coli. Real-time PCR using DNA extracted from FFPE sections was useful but not consistent for identifying the Ck genome in GM, while immunostaining using cross-reactive antisera against four kinds of bacteria was not Ck-specific but was applicable to visualizing bacterial infection within the GM lesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |