The neonicotinoid thiacloprid causes transcriptional alteration of genes associated with mitochondria at environmental concentrations in honey bees
| Autor: | Simon Schmid, Michael Schmid, Karl Fent, Timm Hettich |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Insecticides
Nuclear gene 010504 meteorology & atmospheric sciences Health Toxicology and Mutagenesis Farnesol dehydrogenase Thiazines Oxidative phosphorylation 010501 environmental sciences Biology Mitochondrion Toxicology 01 natural sciences chemistry.chemical_compound Neonicotinoids Tandem Mass Spectrometry Gene expression Animals 0105 earth and related environmental sciences General Medicine Honey bee Bees Thiacloprid Pollution Mitochondria Metabolic pathway Biochemistry chemistry Energy Metabolism Transcriptome Chromatography Liquid |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 266(Pt 1) |
| ISSN: | 1873-6424 |
| Popis: | Thiacloprid is widely used in agriculture and may affect pollinators. However, its molecular effects are poorly known. Here, we report the global gene expression profile in the brain of honey bee foragers assessed by RNA-sequencing. Bees were exposed for 72 h to nominal concentrations of 25 and 250 ng/bee via sucrose solution. Determined residue concentrations by LC-MS/MS were 0.59 and 5.49 ng/bee, respectively. Thiacloprid exposure led to 5 and 71 differentially expressed genes (DEGs), respectively. Nuclear genes encoding mitochondrial ribosomal proteins and enzymes involved in oxidative phosphorylation, as well as metabolism enzymes and transporters were altered at 5.49 ng/bee. Kyoto Encylopedia of Genes and Genomes (KEGG) analysis revealed that mitochondrial ribosome proteins, mitochondrial oxidative phosphorylation, pyrimidine, nicotinate and nicotinamide metabolism and additional metabolic pathways were altered. Among 21 genes assessed by RT-qPCR, the transcript of farnesol dehydrogenase involved in juvenile hormone III synthesis was significantly down-regulated. Transcripts of cyp6a14-like and apolipophorin-II like protein, cytochrome oxidase (cox17) and the non-coding RNA (LOC102654625) were significantly up-regulated at 5.49 ng/bee. Our findings indicate that thiacloprid causes transcriptional changes of genes prominently associated with mitochondria, particularly oxidative phosphorylation. This highlight potential effects of this neonicotinoid on energy metabolism, which may compromise bee foraging and thriving populations at environmentally relevant concentrations. |
| Databáze: | OpenAIRE |
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