Epithelial-Derived Reactive Oxygen Species Enable AppBCX-Mediated Aerobic Respiration of Escherichia coli during Intestinal Inflammation
| Autor: | Lisa Büttner, Wenhan Zhu, Rachael B. Chanin, Madeline P. Smoot, Angel G. Jimenez, Sebastian E. Winter, Savannah J. Taylor, Luisella Spiga, Caroline C. Gillis, Renato L. Santos, Alexandre Arenales, Maria G. Winter, Elizabeth R. Hughes |
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| Rok vydání: | 2020 |
| Předmět: |
gut microbiota dysbiosis
Male Microbial metabolism Inbred C57BL Mice 0302 clinical medicine Intestinal Mucosa Inbred BALB C chemistry.chemical_classification reactive oxygen species 0303 health sciences Oxidase test Mice Inbred BALB C Escherichia coli Proteins Microbiota Bacterial NADPH Oxidase 1 Colitis Aerobiosis Infectious Diseases Medical Microbiology NOX1 Female gut inflammation Infection DNA Bacterial Cellular respiration Immunology Biology Autoimmune Disease Microbiology Article Electron Transport Complex IV 03 medical and health sciences microbial respiration Virology Genetic model Escherichia coli Cytochrome c oxidase Animals intestinal epithelium 030304 developmental biology Inflammation Reactive oxygen species Host Microbial Interactions Animal Prevention Inflammatory Bowel Disease DNA cytochrome oxidase Hydrogen Peroxide Gastrointestinal Microbiome Mice Inbred C57BL Oxygen Disease Models Animal Good Health and Well Being chemistry Disease Models biology.protein Parasitology Digestive Diseases Reactive Oxygen Species 030217 neurology & neurosurgery |
| Zdroj: | Cell Host Microbe Cell host & microbe, vol 28, iss 6 |
| ISSN: | 1934-6069 |
| Popis: | The intestinal epithelium separates host tissue and gut-associated microbial communities. During inflammation, the host releases reactive oxygen and nitrogen species as an antimicrobial response. The impact of these radicals on gut microbes is incompletely understood. We discovered that the cryptic appBCX genes, predicted to encode a cytochrome bd-II oxidase, conferred a fitness advantage for E.coli in chemical and genetic models of non-infectious colitis. This fitness advantage was absent in mice that lacked epithelial NADPH oxidase 1 (NOX1) activity. In laboratory growth experiments, supplementation with exogenous hydrogen peroxide enhanced E.coli growth through AppBCX-mediated respiration in a catalase-dependent manner. We conclude that epithelial-derived reactive oxygen species are degraded in the gut lumen, which gives rise to molecular oxygen that supports the aerobic respiration of E.coli. This work illustrates how epithelial host responses intersect with gut microbial metabolism in the context of gut inflammation. |
| Databáze: | OpenAIRE |
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