Determination and quantitation of sildenafil and its major metabolite in the breast milk of a lactating woman
| Autor: | Nicholas Schramek, Uwe Wollein, Jochen Hardt, Bernd Schech |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Sildenafil
Metabolite Clinical Biochemistry Analytical chemistry Pharmaceutical Science Breast milk Tandem mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Sildenafil Citrate Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Lactation Drug Discovery medicine Humans Familial Primary Pulmonary Hypertension Sample preparation Human breast milk Spectroscopy Chromatography Milk Human 010401 analytical chemistry respiratory tract diseases 0104 chemical sciences medicine.anatomical_structure chemistry cardiovascular system Female Chromatography Liquid |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 120:100-105 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2015.12.006 |
| Popis: | A heavily pregnant woman was treated with Revatio(®) (Sildenafil) against idiopathic pulmonary arterial hypertension, prior to and after her accouchement. To investigate the transfer of sildenafil into breast milk and its metabolism shortly before breastfeeding to the neonatal, a new analytical method was developed and validated, using liquid chromatography tandem mass spectrometry. Additionally, while using linear ion trap scan mode experiments, further metabolites could be identified. Sample preparation was carried out, using solid-phase extraction. For quantification of sildenafil and its major metabolite N-desmethylsildenafil, sildenafil-d8 was used as an internal standard. Within a time frame of 17h covering two Revatio(®) intakes and three breast milk samplings, a concentration range from 1.64 to 4.49ng/ml (sildenafil) and from 1.18 to 1.82ng/ml (N-desmethylsildenafil) could be observed. The current method proved to be accurate and precise in a very low concentration range and establishes the first reported determination of sildenafil and N-desmethylsildenafil in human breast milk. |
| Databáze: | OpenAIRE |
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