Peroxiredoxin 1 promoted tumor metastasis and angiogenesis in colorectal cancer
| Autor: | Xiangyu Sun, Huan-Xi Li, Shi-Ming Yang, Zhen-Yu Wang, Qing Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male MMP2 Colorectal cancer Angiogenesis MMP9 Biology Pathology and Forensic Medicine Metastasis 03 medical and health sciences HT29 Cells 0302 clinical medicine Cell Movement Cell Line Tumor medicine Biomarkers Tumor Humans Aged Cell Proliferation Tube formation Aged 80 and over Neovascularization Pathologic Cell Biology Peroxiredoxins Middle Aged medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic Vascular endothelial growth factor A 030104 developmental biology 030220 oncology & carcinogenesis Lymphatic Metastasis Colonic Neoplasms Cancer research Female Colorectal Neoplasms |
| Zdroj: | Pathology, research and practice. 214(5) |
| ISSN: | 1618-0631 |
| Popis: | Peroxiredoxin1 (Prdx1) is a member of the PrdxS family, and it regulates cellular signaling and differentiation. The role of Prdx1in colorectal cancer (CRC) remains unclear. In this study, we investigated the relevance of Prdx1 in the metastasis and angiogenesis of CRC. The expression of Prdx1 in 60 cases human CRC tissues was detected through immunohistochemistry. The tumors that highly expressed Prdx1 (42/60) exhibited higher tumor grade and lymph node metastasis than those with low expression of Prdx1 (18/60) (p < 0.05). Kaplan-Meier survival analysis showed that the survival time of thePrdx1-positive group was shorter than that of thePrdx1-negative group (p = 0.046).Moreover, a statistically significant correlation was observed between the Prdx1 expression and microvessel density (p = 0.004). Transwell migration assay revealed that Prdx1 was down-regulated in the CRC cell line HCT116, thereby suppressing the invasion and migration capacities of tumor cells, whereas Prdx1was up-regulated in HT29 cells, thereby increasing the invasion and migration capacities of tumor cells. The tube formation capacity of human umbilical vein endothelial cells cultured in 3D medium was increased after conditioned medium from overexpressed Prdx1cancer cells was added relative to that when down-regulated Prdx1 cell medium was added (p < 0.05). In addition, up-regulated Prdx1 increased the protein expression of MMP2, MMP9, and VEGFA. These data suggested that Prdx1 expression predicted poor prognosis by regulating the tumor metastasis and angiogenesis of CRC. Therefore, Prdx1 may serve as a potential therapeutic target. |
| Databáze: | OpenAIRE |
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