Photodynamic inactivation of influenza and herpes viruses by hematoporphyrin
| Autor: | N. L. Shipkowitz, Jian-Hua Mao, M. Perlin, R. G. Duff, E. R. Otis |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Light
viruses Orthomyxoviridae Guanosine Monophosphate Chick Embryo Biology medicine.disease_cause Virus Replication Herpesviridae Virus Microbiology chemistry.chemical_compound Mice RNA Transfer Virology Influenza A virus medicine Animals Simplexvirus Vero Cells Cells Cultured Nucleic Acid Synthesis Inhibitors Pharmacology Hematoporphyrin Embryonated RNA DNA-Directed RNA Polymerases Fibroblasts biology.organism_classification In vitro Hematoporphyrins chemistry Rabbits |
| Zdroj: | Antiviral research. 7(1) |
| ISSN: | 0166-3542 |
| Popis: | Hematoporphyrin (HP), at concentrations as low as 0.5 μg/ml, was found to inhibit the in vitro replication of influenza A and herpes simplex viruses, but not of several other viruses. The effect required exposure of the viruses or cells to visible light and was demonstrable when HP was administered shortly before virus inoculation or during the infection. In studies on the mechanism of action of HP, we found that in the presence of light, HP caused decomposition of GMP but not of various other nucleosides. It caused breakdown of yeast tRNA and inhibited polymerization of RNA and DNA by influenza virus and HSV-1-specific polymerases as well as some other polymerases isolated from bacterial and mammalian sources. Protective effects of HP and light were demonstrable in embryonated eggs infected with the WSN and PR8 strains of influenza A virus and in mice infected with the WSN strain. HSV-1-induced keratitis in rabbits and HSV-2-induced dermatitis in mice were not responsive to HP treatment. |
| Databáze: | OpenAIRE |
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