The attachment of Schistosoma mansoni cercariae to human skin cells
| Autor: | A. Bartlett, N. Khammo, Philip J. Whitfield, R. H. Clothier |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Keratinocytes
Cell type Pathology medicine.medical_specialty Cellular differentiation Human skin Foreskin parasitic diseases medicine Skin equivalent Animals Humans Microscopy Phase-Contrast Skin integumentary system biology Cell Differentiation Schistosoma mansoni Fibroblasts biology.organism_classification Schistosomiasis mansoni Cell biology Infectious Diseases medicine.anatomical_structure Microscopy Fluorescence Cell culture Microscopy Electron Scanning Animal Science and Zoology Parasitology Keratinocyte |
| Zdroj: | Parasitology. 124(Pt 1) |
| ISSN: | 0031-1820 |
| Popis: | Most of our knowledge about the process of penetration of skin, by cercariae of Schistosoma mansoni, has been gained from studies carried out in vivo with laboratory animals. Human skin is significantly different from that of other animals but there are obvious practical difficulties in directly studying attachment and penetration with human skin. Techniques have been developed which enable a 3-dimensional ‘skin equivalent’ to be grown in tissue culture, made from different types of human skin cells. The aim of the present study was to investigate cercarial interactions with confluent cultures of the individual skin cell types that make up normal human skin and which will be used to construct a multi-component model. Cercariae behaved differently towards the various cell types tested. They responded least to monolayers of endothelial cells and most to primary keratinocytes, derived from human foreskin and differentiated at an air/liquid interface. This study demonstrates, therefore, that cercariae are capable of distinguishing between different types of skin cells and they preferentially attach to differentiated cells which form the epidermis. |
| Databáze: | OpenAIRE |
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