Chrysin-induced ERK1/2 Phosphorylation Enhances the Sensitivity of Human Hepatocellular Carcinoma Cells to Sorafenib
| Autor: | Pei Hsuan Chien, Yung Jun Hung, Lei Chin Chen, Hsiao Lin Pan, Yun Ju Chen, Yi Ping Hsiang, Ching Ting Wei |
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| Rok vydání: | 2019 |
| Předmět: |
Sorafenib
Cancer Research Carcinoma Hepatocellular Abcg2 Cell Survival 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine Cell Line Tumor Antineoplastic Combined Chemotherapy Protocols medicine ATP Binding Cassette Transporter Subfamily G Member 2 Humans Viability assay Chrysin Phosphorylation Extracellular Signal-Regulated MAP Kinases Protein kinase A neoplasms Flavonoids Dose-Response Relationship Drug biology Chemistry Kinase Liver Neoplasms Drug Synergism Hep G2 Cells General Medicine medicine.disease digestive system diseases Neoplasm Proteins Oncology 030220 oncology & carcinogenesis Hepatocellular carcinoma biology.protein Cancer research medicine.drug |
| Zdroj: | Anticancer Research. 39:695-701 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13165 |
| Popis: | Background/aim Sorafenib is now standard treatment for advanced hepatocellular carcinoma (HCC). However, therapeutic efficacy is not as good as was predicted. Many efforts are being made to improve HCC sensitivity to sorafenib. Our previous study demonstrated that co-treatment with chrysin enhanced sorafenib sensitivity through inhibition of ATP-binding cassette super-family G member 2 (ABCG2). Whether there is another mechanism other than inhibition of ABCG2 underlying chrysin-mediated synergistic effect is still not completely elucidated. Materials and methods Phosphorylation of extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) was examined by western blot. Cell viability was examined by crystal violet staining. The importance of ERK1/2 phosphorylation was assessed by overexpression and blockage of mitogen-activated protein kinase kinase 1 (MEK1). Results Chrysin induced sustained ERK1/2 phosphorylation of HCC cells in both time- and dose-dependent manners. Overexpression of MEK1 enhanced, whereas blockage of MEK1 led to loss of chrysin-synergized sorafenib effect, through modulating ERK1/2 phosphorylation level. Conclusion These results identify another novel mechanism underlying chrysin-mediated synergistic effect on sorafenib activity in HCC cells. |
| Databáze: | OpenAIRE |
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