Differentiation in a transplantable rat tumour maintained in organ culture
| Autor: | M. L. Ellison, G.C. Easty, E.J. Ambrose |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Organ culture Mice Tumour tissue chemistry.chemical_compound Culture Techniques medicine Animals Sarcoma 180 Kidney Cell Differentiation Membranes Artificial Neoplasms Experimental Cell Biology Embryonic Tissue Embryonic stem cell Kidney Neoplasms Rats Information coding medicine.anatomical_structure chemistry Genetic Code Immunology Cell Division Neoplasm Transplantation Nutrient agar |
| Zdroj: | Experimental Cell Research. 55:198-204 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(69)90481-9 |
| Popis: | Experiments were carried out to test the hypothesis that tumour cells still retain genetic information coding for specific differentiated characteristics while the characteristics may not themselves be apparent in the tumour tissue. Tumour samples supported on Millipore filter rafts were organ cultured on nutrient agar in combination with embryonic “inducing tissues” for up to 3 weeks, or for up to 12 weeks without embryonic tissue. Characterisable morphological changes were found in one of the tumours tested—a transplantable rat tumour which originated spontaneously in the kidney. Although a highly anaplastic tumour, it formed tubules under the conditions of culture. There was no evidence to suggest that embryonic tissues could “induce” specific differentiations, but it was concluded that the genetic information for a differentiated characteristic was not irreversibly lost in this example of an anaplastic tumour. |
| Databáze: | OpenAIRE |
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