Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study

Autor: Raquel Sainz-Urruela, Francisco O'Valle, Per Anderson, Miguel Padial-Molina, Dario Abril-Garcia, Juan G. de Buitrago, Pablo Galindo-Moreno
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Homeobox protein NANOG
musashi-1
Stromal cell
RUNX2
osteogenic differentiation
Context (language use)
Core Binding Factor Alpha 1 Subunit
Nerve Tissue Proteins
Biology
Periostin
Catalysis
Article
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
0302 clinical medicine
SOX2
bone regeneration
Osteogenesis
Humans
RNA
Messenger
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Cells
Cultured
periostin
Dental follicle
Mouth
Organic Chemistry
Mesenchymal stem cell
RNA-Binding Proteins
Cell Differentiation
Mesenchymal Stem Cells
030206 dentistry
General Medicine
Computer Science Applications
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Cancer research
bone healing
mesenchymal stromal cells
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 9
Digibug. Repositorio Institucional de la Universidad de Granada
instname
International Journal of Molecular Sciences, Vol 20, Iss 9, p 2171 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20092171
Popis: Supplementary materials can be found at https://www.mdpi.com/1422-0067/20/9/2171/s1
Background: Musashi-1 (MSI1) is a negative regulator of mesenchymal stromal cell (MSC) differentiation which in turn favors cell proliferation. However, little is known about its expression by MSC from the oral cavity and in the context of osteogenic differentiation. Aim: The aim of this study was to analyze the expression of MSI1 in the context of osteogenic differentiation of MSC derived from the oral cavity. Material/methods: For this in vitro study, MSC were isolated from six different origins of the oral cavity. They were extensively characterized in terms of proliferative and clonogenicity potential, expression of stemness genes (MYC, NANOG, POU5F1, and SOX2), expression of surface markers (CD73, CD90, CD105, CD14, CD31, CD34, and CD45) and adipo-, chondro- and osteogenic differentiation potential. Then, osteogenic differentiation was induced and the expression of MSI1 mRNA and other relevant markers of osteogenic differentiation, including RUNX2 and Periostin, were also evaluated. Results: Cell populations from the alveolar bone (pristine or previously grafted with xenograft), dental follicle, dental germ, dental pulp, and periodontal ligament were obtained. The analysis of proliferative and clonogenicity potential, expression of the stemness genes, expression of surface markers, and differentiation potential showed similar characteristics to those of previously published MSC from the umbilical cord. Under osteogenic differentiation conditions, all MSC populations formed calcium deposits and expressed higher SPARC. Over time, the expression of MSI1 followed different patterns for the different MSC populations. It was not significantly different than the expression of RUNX2. In contrast, the expression of MSI1 and POSTN and RUNX2 were statistically different in most MSC populations. Conclusion: In the current study, a similar expression pattern of MSI1 and RUNX2 during in vitro osteogenic differentiation was identified.
The authors of this investigation were partially supported by Research Groups #CTS-138 (F.O.) and #CTS-1028 (M.P.-M., P.G.-M.) (Junta de Andalucía, Spain), a grant from MIS Implant Technologies Ltd. (M.P.-M., D.A.-G., P.G.-M.), the Youth Employment Initiative (YEI) from the European Commission (R.S.-U.), and the Instituto de Salud Carlos III, Spain (www.isciii.es) and Fondo Europeo de Desarrollo Regional (FEDER, from the European Union), through the research grants PI15/00794 and CPII15/00032 (P.A).
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje