Safety evaluation of β-glucanase derived from trichoderma reesei: Summary of toxicological data

Autor: A.C.M. Schoenmakers, T.M.M. Coenen, Hans Verhagen
Přispěvatelé: Centraal Instituut voor Voedingsonderzoek TNO
Rok vydání: 1995
Předmět:
Male
Salmonella typhimurium
barlican
Sus scrofa
Administration
Oral
Pharmacology
Dermatitis
Contact
Toxicology
Oryctolagus cuniculus
Median lethal dose
skin irritation
Cavia porcellus
Cricetinae
rat
Chronic toxicity
Trichoderma reesei
Trichoderma
biology
Inhalation
beta-Glucosidase
article
Hypocrea jecorina
General Medicine
Environmental exposure
unclassified drug
female
Toxicity
Hamsters
Rabbits
eye irritation
No-observed-adverse-effect level
chicken
Feed additive
animal experiment
Guinea Pigs
rabbit
Gallus gallus
CHO Cells
Lethal Dose 50
Toxicity Tests
Animals
Animalia
controlled study
skin sensitization
Rats
Wistar
Biological Products
No-Observed-Adverse-Effect Level
nonhuman
Animal
Mutagenicity Tests
mutagenicity
Glucan 1
3-beta-Glucosidase
Environmental Exposure
beta glucan hydrolase
biology.organism_classification
Animal Feed
Rats
Food Additives
Chickens
chronic toxicity
guinea pig
Food Science
Zdroj: Food and Chemical Toxicology, 10, 33, 859-866
ISSN: 0278-6915
DOI: 10.1016/0278-6915(95)00052-4
Popis: Barlican, a beta-glucanase enzyme obtained from Trichoderma reesei, was produced by a fermentation process and subjected to a series of toxicological tests to document its safety for use as a feed additive. The enzyme product was examined for general oral toxicity, inhalation toxicity, irritation to eye and skin, skin sensitization and mutagenic potential. An extensive literature search on the production organism was also conducted. Furthermore, safety for target species was assessed in a 28-day oral toxicity study with broilers. A strong skin-sensitizing potential of the beta-glucanase enzyme was detected, but no other evidence of oral or inhalation toxicity, mutagenic potential, eye or skin irritancy was found. Feeding of the beta-glucanase enzyme at dietary levels up to 10,000 ppm in the 90-day subchronic toxicity study in rats did not induce noticeable signs of toxicity. In addition, no adverse effects were observed when broiler chicks were fed dietary concentrations of the beta-glucanase enzyme up to eight times the daily recommended dose. It is therefore concluded that this beta-glucanase preparation is safe for use in feed of the intended target species. However, some occupational health precautions should be taken to avoid skin contact and inhalation, as is the case for almost all enzyme proteins.
Databáze: OpenAIRE
Externí odkaz: