Spermatogenesis and gonadal cycle in male Tamoya haplonema and Chiropsalmus quadrumanus (Cnidaria, Cubozoa)
| Autor: | Jimena García-Rodríguez, Gisele Rodrigues Tiseo, Fernando José Zara, Cheryl Lewis Ames, Antonio C. Marques, André C. Morandini |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Smithsonian Institution |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Cnidaria Chiropsalmus quadrumanus endocrine system Gonad Histology 010607 zoology Mesoglea 010603 evolutionary biology 01 natural sciences Box jellyfish medicine Histochemistry biology urogenital system Anatomy biology.organism_classification CNIDARIA Sperm medicine.anatomical_structure Animal Science and Zoology Acrosomal vesicle Development of the gonads Spermatogenesis |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2019-10-06T16:15:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Spermatogenesis has been little studied in cubozoans. This work describes spermatogenesis of two cubozoan species from Brazil, Tamoya haplonema and Chiropsalmus quadrumanus, using light microscopy and histochemical techniques to highlight and contrast stages of the gonadal cycle. Specimens were collected by hand at the water surface or with shrimp trawls in three areas in the state of São Paulo (SE Brazil): Cananéia lagoon estuarine system (south), Santos and São Vicente Bay (central), and São Sebastião and Ubatuba (north). Male box jellyfish gonads are located between the mesoglea and gastrodermis in a thin and independent layer, organized in elongated follicles, macroscopically resembling a fingerprint pattern. After subsampling, serial sections were performed on male gonad tissue. The resulting slides were stained with hematoxylin and eosin and methods for staining proteins, acidic materials and neutral polysaccharides. Light microscopy revealed that the follicle wall of the male gonads of both cubozoan species is composed of spermatogonia. Spermatocytes are found peripherally near the follicle wall, while spermatids and spermatozoa are located towards the center. Sperm release occurs through rupture of the follicular wall into the gastric pockets. Four stages of gonad development were documented for T. haplonema and three for C. quadrumanus. The sperm of C. quadrumanus has a rounded head while in T. haplonema the sperm head is conical with an elongated apical projection. In both species, there was no positive histochemical reaction for putative acrosomal vesicles precursors. Departamento de Zoologia Instituto de Biociências Universidade de São Paulo, R. Matão trav. 14, n.101 Departamento de Biologia Aplicada Invertebrate Morphology Laboratory (IML) Aquaculture Center (CAUNESP) and Instituto de Estudos Avançados do Mar (IEAMar) Univ. Estadual Paulista (UNESP), Via de Acesso Prof. Donato Castellane, S/N Department of Invertebrate Zoology National Museum of Natural History Smithsonian Institution Centro de Biologia Marinha Universidade de São Paulo, Rodovia Manoel Hypólito do Rego, km. 131.5 Departamento de Biologia Aplicada Invertebrate Morphology Laboratory (IML) Aquaculture Center (CAUNESP) and Instituto de Estudos Avançados do Mar (IEAMar) Univ. Estadual Paulista (UNESP), Via de Acesso Prof. Donato Castellane, S/N FAPESP: 2012/19080-1 FAPESP: 2014/08785-1 CNPq: 304961/2016-7 CNPq: 309995/2017-5 CAPES: ACMa 23038.004309/2014–51 CNPq: ACMa 445444/2014-2 CNPq: ACMo 301039/2013-5 |
| Databáze: | OpenAIRE |
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