Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection
| Autor: | Claudia Rengucci, Giulia De Maio, Francesca Benzi, Maura Menghi, Daniele Calistri |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology Adult Data Analysis medicine.medical_specialty Colorectal cancer General Chemical Engineering Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Fluorescence 03 medical and health sciences chemistry.chemical_compound Feces 0302 clinical medicine Risk Factors Internal medicine medicine Prevalence Humans Stool dna Dna integrity General Immunology and Microbiology Crc screening business.industry General Neuroscience Fecal occult blood Temperature DNA Middle Aged Reference Standards medicine.disease genomic DNA 030104 developmental biology Real-time polymerase chain reaction chemistry 030220 oncology & carcinogenesis Occult Blood business Colorectal Neoplasms |
| Zdroj: | Journal of visualized experiments : JoVE. (160) |
| ISSN: | 1940-087X |
| Popis: | Nowadays, stool DNA can be isolated and analyzed by several methods. The long fragments of DNA in stool can be detected by a qPCR assay, which provides a reliable probability of the presence of pre-neoplastic or neoplastic colorectal lesions. This method, called fluorescence long DNA (FL-DNA), is a fast, non-invasive procedure that is an improvement upon the primary prevention system. This method is based on evaluation of fecal DNA integrity by quantitative amplification of specific targets of genomic DNA. In particular, the evaluation of DNA fragments longer than 200 bp allows for detection of patients with colorectal lesions with very high specificity. However, this system and all currently available stool DNA tests present some general issues that need to be addressed (e.g., the frequency at which tests should be carried out and optimal number of stool samples collected at each timepoint for each individual). However, the main advantage of FL-DNA is the possibility to use it in association with a test currently used in the CRC screening program, known as the immunochemical-based fecal occult blood test (iFOBT). Indeed, both tests can be performed on the same sample, reducing costs and achieving a better prediction of the eventual presence of colorectal lesions. |
| Databáze: | OpenAIRE |
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