Analysis of sequence variations in the suppressor of cytokine signaling (SOCS)-3 gene in extremely obese children and adolescents
| Autor: | Johannes Hebebrand, Anke Hinney, André Scherag, Katja Hölter, Anne-Kathrin Wermter, Wolfgang Siegfried, Hanspeter Goldschmidt |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male lcsh:Internal medicine lcsh:QH426-470 Adolescent Genotype medicine.medical_treatment Regulator Medizin Suppressor of Cytokine Signaling Proteins Biology Suppressor of cytokine signalling Polymorphism Single Nucleotide Linkage Disequilibrium law.invention Gene Frequency law Germany Genetics medicine Odds Ratio Coding region Humans Genetics(clinical) SOCS3 Obesity lcsh:RC31-1245 Child SOCS2 Genetics (clinical) Alleles DNA Primers Suppressor of cytokine signaling 1 lcsh:Genetics Cytokine Suppressor of Cytokine Signaling 3 Protein Case-Control Studies Immunology Suppressor Female Research Article |
| Zdroj: | BMC Medical Genetics BMC Medical Genetics, Vol 8, Iss 1, p 21 (2007) |
| Popis: | Background The suppressor of cytokine signaling (SOCS)-3 is a negative feedback regulator of cytokine signaling and also influences leptin signaling. We investigated association of variations in the coding sequence and promoter region of SOCS3 with extreme obesity in German children and adolescents. Methods An initial screen for sequence variations in 181 extremely obese children and adolescents and 188 healthy underweight adults revealed two previously reported single nucleotide polymorphisms (SNPs) in the SOCS3 5' region: -1044 C>A (numbering refers to bases upstream of ATG in exon 2) within a predicted STAT3 binding element and -920 C>A (rs12953258, for numbering, see above). Results We did not detect significant differences in allele or genotype frequencies for any of these SNPs between the analysed study groups (all nominal p > 0.2). In addition, we performed a pedigree transmission disequilibrium test (PDT) for the SNP -1044 C>A in families comprising 703 obese children and adolescents, 281 of their obese siblings and both biological parents. The PDT revealed no transmission disequilibrium (nominal p > 0.05). Conclusion In conclusion, our data do not suggest evidence for a major role of the respective SNPs in SOCS3 in the pathogenesis of extreme obesity in our study groups. |
| Databáze: | OpenAIRE |
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