Production of Methionol from 3-Methylthiopropionaldehyde by Catalysis of the Yeast Alcohol Dehydrogenase Adh4p
| Autor: | Xu Ruixin, Hui Wang, Qi Wang, Yiping Wu, Sheng Yin, Che Yixin, Julien Boutet, Huaqing Yang, Robert Huet, Chengtao Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Saccharomyces cerevisiae Proteins Propanols Saccharomyces cerevisiae Gene Expression Sulfides medicine.disease_cause 01 natural sciences Enzyme catalysis chemistry.chemical_compound medicine Escherichia coli Aroma compound Fermentation in food processing Alcohol dehydrogenase Aldehydes biology 010401 analytical chemistry Alcohol Dehydrogenase General Chemistry biology.organism_classification 0104 chemical sciences chemistry Biochemistry ADH4 Fermentation biology.protein Biocatalysis General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Journal of agricultural and food chemistry. 68(16) |
| ISSN: | 1520-5118 |
| Popis: | Methionol is a sulfur-containing aroma compound that contributes to the flavors of fermented foods. In this work, a novel method for methionol production was established using 3-methylthiopropionaldehyde (MMP) and alcohol dehydrogenase (ADH). First, expression of seven ADH genes was analyzed in yeast fermentation added with MMP. Only ADH4 displayed an evident increased expression in response to MMP. ADH4 was then overexpressed in Saccharomyces cerevisiae S288c and Escherichia coli BL21. The recombinant yeast strain S4 produced more methionol than control strain in MMP fermentation. Furthermore, 0.55 g/L 42 kDa Adh4p was prepared from E. coli by induced expression and purification. A fed-batch catalysis system was finally established to produce methionol from MMP by Adh4p. In 10 h of continuous catalysis, the conversion rate of MMP remained 80-95%, and a final yield of 0.2 g/L methionol was achieved. This work proposed a novel method for methionol production by enzymatic catalysis with a potential application prospect in industry. |
| Databáze: | OpenAIRE |
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