High level and enantioselective production of L-phenylglycine from racemic mandelic acid by engineered Escherichia coli using response surface methodology
| Autor: | Xiang Li, Lin-Feng Wang, Jian-He Xu, Yuan-Yuan Jia, Kan Yunchao, Lun-Guang Yao, Tang Cunduo, Hong-Ling Shi |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glycine Bioengineering Dehydrogenase 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Catalysis 03 medical and health sciences chemistry.chemical_compound Industrial Microbiology Bacterial Proteins 010608 biotechnology Escherichia coli chemistry.chemical_classification Chemistry digestive oral and skin physiology Mandelate racemase Enantioselective synthesis Stereoisomerism Mandelic acid Combinatorial chemistry Amino acid Kinetics 030104 developmental biology Biocatalysis Yield (chemistry) Mandelic Acids Biotechnology Plasmids |
| Zdroj: | Enzyme and microbial technology. 136 |
| ISSN: | 1879-0909 |
| Popis: | L-Phenylglycine (L-PHG) is a member of unnatural amino acids, and becoming more and more important as intermediate for pharmaceuticals, food additives and agrochemicals. However, the existing synthetic methods for L-PHG mainly rely on toxic cyanide chemistry and multistep processes. To provide green, safe and high enantioselective alternatives, we envisaged cascade biocatalysis for the one-pot synthesis of L-PHG from racemic mandelic acid. A engineered E. coli strain was established to co-express mandelate racemase, D-mandelate dehydrogenase and L-leucine dehydrogenase and catalyze a 3-step reaction in one pot, enantioselectively transforming racemic mandelic acid to give L-PHG (e.e. >99 %). After the conditions for biosynthesis of L-PHG optimized by response surface methodology, the yield and space-time yield of L-PHG can reach 87.89 % and 79.70 g·L−1·d−1, which was obviously improved. The high-yielding and enantioselective synthetic methods use cheap and green reagents, and E. coli whole-cell catalysts, thus providing green and useful alternative methods for manufacturing L-PHG. |
| Databáze: | OpenAIRE |
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