The dual specificity phosphatase Cdc25C is a direct target for transcriptional repression by the tumor suppressor p53
| Autor: | James J. Manfredi, Selvon St. Clair |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Mutation
Sp1 transcription factor Models Genetic Transcription Genetic Mutant Cell Cycle Proteins Cell Biology Biology medicine.disease_cause Molecular biology Repressor Proteins Downregulation and upregulation Transcription (biology) medicine Animals Humans cdc25 Phosphatases Binding site Tumor Suppressor Protein p53 Promoter Regions Genetic Molecular Biology Psychological repression Developmental Biology P53 binding |
| Zdroj: | Cell cycle (Georgetown, Tex.). 5(7) |
| ISSN: | 1551-4005 |
| Popis: | The cdc25C gene has been shown to be a novel target for transcriptional downregulation by p53. Two independent mechanisms contribute to the p53-dependent repression of the cdc25C gene. First, an element in the cdc25C promoter consisting of a binding site for p53 plus an adjacent 8 base pairs confers p53-dependent repression. Mutation of either the p53 binding site or the adjacent 8 bp sequence abolishes this effect. The element conferring p53-dependent repression also contains a binding site for the transcription factor Sp1 and a mutant p53 protein that retains the ability to interact with the p53-binding site is defective in mediating repression. Second, a minimal promoter lacking the p53 binding site but containing a previously characterized CDE/CHR element is also repressed by p53. This repression is abrogated when a 5 bp mutation is introduced in the CHR sequence. These results support a model for p53 downregulating cdc25C expression, in part, by direct binding to a promoter element that is likely to require cooperation with an additional cellular factor. |
| Databáze: | OpenAIRE |
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