| Autor: |
Nkom F Ntumvi, Valantine Ngum Ndze, Amethyst Gillis, Joseph Le Doux Diffo, Ubald Tamoufe, Jean-Michel Takuo, Moctar M M Mouiche, Julius Nwobegahay, Matthew LeBreton, Anne W Rimoin, Bradley S Schneider, Corina Monagin, David J McIver, Sanjit Roy, James A Ayukekbong, Karen E Saylors, Damien O Joly, Nathan D Wolfe, Edward M Rubin, Christian E Lange |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Virus evolution. 8(1) |
| ISSN: |
2057-1577 |
| Popis: |
Zoonotic spillover of animal viruses into human populations is a continuous and increasing public health risk. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the global impact of emergence. Considering the history and diversity of coronaviruses (CoVs), especially in bats, SARS-CoV-2 will likely not be the last to spillover from animals into human populations. We sampled and tested wildlife in the Central African country Cameroon to determine which CoVs are circulating and how they relate to previously detected human and animal CoVs. We collected animal and ecological data at sampling locations and used family-level consensus PCR combined with amplicon sequencing for virus detection. Between 2003 and 2018, samples were collected from 6,580 animals of several different orders. CoV RNA was detected in 175 bats, a civet, and a shrew. The CoV RNAs detected in the bats represented 17 different genetic clusters, coinciding with alpha (n = 8) and beta (n = 9) CoVs. Sequences resembling human CoV-229E (HCoV-229E) were found in 40 Hipposideridae bats. Phylogenetic analyses place the human-derived HCoV-229E isolates closest to those from camels in terms of the S and N genes but closest to isolates from bats for the envelope, membrane, and RNA-dependent RNA polymerase genes. The CoV RNA positivity rate in bats varied significantly (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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