CKAMP44 modulates integration of visual inputs in the lateral geniculate nucleus
| Autor: | Kevin Allen, Jakob von Engelhardt, Muhammad Aslam, Xufeng Chen, Tim Gollisch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
cytology [Geniculate Bodies] physiology [Retina] General Physics and Astronomy Gating Synaptic Transmission 0302 clinical medicine Desensitization (telecommunications) metabolism [Geniculate Bodies] physiology [Geniculate Bodies] Receptor lcsh:Science genetics [Nerve Tissue Proteins] Neurons Mice Knockout Multidisciplinary medicine.diagnostic_test musculoskeletal neural and ocular physiology Geniculate Bodies physiology [Neurons] cytology [Retina] metabolism [Neurons] Excitatory postsynaptic potential Female ddc:500 metabolism [Retina] genetics [Synaptic Transmission] physiology [Excitatory Postsynaptic Potentials] Science Nerve Tissue Proteins AMPA receptor Biology Lateral geniculate nucleus Retinal ganglion Retina Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences medicine Electroretinography Animals Receptors AMPA metabolism [Nerve Tissue Proteins] CKAMP44 protein mouse metabolism [Receptors AMPA] Excitatory Postsynaptic Potentials General Chemistry Mice Inbred C57BL 030104 developmental biology nervous system physiology [Synaptic Transmission] Synapses lcsh:Q physiology [Synapses] Neuroscience 030217 neurology & neurosurgery Photic Stimulation |
| Zdroj: | Nature Communications 9(1), 261 (2018). doi:10.1038/s41467-017-02415-1 Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018) Nature Communications |
| DOI: | 10.1038/s41467-017-02415-1 |
| Popis: | Relay neurons in the dorsal lateral geniculate nucleus (dLGN) receive excitatory inputs from retinal ganglion cells (RGCs). Retinogeniculate synapses are characterized by a prominent short-term depression of AMPA receptor (AMPAR)-mediated currents, but the underlying mechanisms and its function for visual integration are not known. Here we identify CKAMP44 as a crucial auxiliary subunit of AMPARs in dLGN relay neurons, where it increases AMPAR-mediated current amplitudes and modulates gating of AMPARs. Importantly, CKAMP44 is responsible for the distinctive short-term depression in retinogeniculate synapses by reducing the rate of recovery from desensitization of AMPARs. Genetic deletion of CKAMP44 strongly reduces synaptic short-term depression, which leads to increased spike probability of relay neurons when activated with high-frequency inputs from retinogeniculate synapses. Finally, in vivo recordings reveal augmented ON- and OFF-responses of dLGN neurons in CKAMP44 knockout (CKAMP44−/−) mice, demonstrating the importance of CKAMP44 for modulating synaptic short-term depression and visual input integration. The function of receptor desensitization in vivo is not well understood. Here, the authors show that deletion of CKAMP44, an AMPAR auxiliary protein that modulates desensitization of AMPAR currents, affects synaptic facilitation at retinogeniculate synapses and visually-evoked firing in awake mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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