Mutational analysis of the internal membrane proximal domain of the HIV glycoprotein C-terminus
Autor: | Tanya Pfeiffer, Heiner Schaal, Marek Widera, Valerie Bosch, Steffen Erkelenz |
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Rok vydání: | 2013 |
Předmět: |
Gene Expression Regulation
Viral Env incorporation HIV Env C-terminus viruses DNA Mutational Analysis Biology Exon In vivo Virology Animals Amino Acid Sequence RNA Messenger Conserved Sequence chemistry.chemical_classification Reverse Transcriptase Polymerase Chain Reaction env Gene Products Human Immunodeficiency Virus Nucleic acid sequence HIV Rev-independent Env expression HIV infection Phenotype Molecular biology Protein Structure Tertiary Amino acid Alternative Splicing Membrane Amino Acid Substitution Biochemistry chemistry Cytoplasm Mutation RNA Viral Conserved region Function (biology) |
Zdroj: | Virology. 440:31-40 |
ISSN: | 0042-6822 |
Popis: | This study focuses on the long stretch of highly conserved amino acids in the membrane proximal part of the HIV-1 cytoplasmic tail (Env amino acids (aa) 706–718) upstream of the overlap with the tat and rev second coding exons. Changes in Env aa 713 and 715, although they did not affect Env function, abrogated replicative spread. Other amino acid substitutions, i.e., 706–712, 714 and 716, despite their conservation, did not result in defective replicative phenotypes even in primary peripheral blood lymphocytes. Our results point to their involvement in presently unrecognized essential Env functions pertinent only in in vivo. Interestingly, changes in the codons for residues 717–718 as well as some mutations in residues 714–716 abrogated Gag expression but still allowed expression of functional Env in a rev-independent manner. This could be due to the inactivation of a rev-regulated negative element within the respective nucleotide sequence (8354–8368). |
Databáze: | OpenAIRE |
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