| Autor: |
Kaspar Podgorski, Johannes Alexander Müller, Abbas Kazemipour, Ariana N. Tkachuk, Loren L. Looger, Nelson Rebola, Jonathan S. Marvin, David Fitzpatrick, Huan Bao, Justin P. Little, Samuel S.-H. Wang, Benjamin Scholl, Francisco José Urra Quiroz, David A. DiGregorio, Susanne Schoch, Dirk Dietrich, Daniel E. Wilson, Adam W. Hantman, Edwin R. Chapman |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| DOI: |
10.1101/235176 |
| Popis: |
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter; can detect sub-micromolar to millimolar concentrations of glutamate; and have blue, green or yellow emission profiles. These variants allow in vivo imaging where original-iGluSnFR was too dim, reveal glutamate transients at individual spine heads, and permit kilohertz imaging with inexpensive, powerful fiber lasers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
